Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Waves of sumoylation support transcription dynamics during adipocyte differentiation

ABSTRACT: Waves of sumoylation support transcription dynamics during adipocyte differentiation

PROVIDER: PRJNA703951 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR13762800.fastq.gz	Fastqsanger.gz
	SRR13762801.fastq.gz	Fastqsanger.gz
	SRR13762802.fastq.gz	Fastqsanger.gz
	SRR13762803.fastq.gz	Fastqsanger.gz
	SRR13762804.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 44

Similar Datasets

Waves of sumoylation support transcription dynamics during adipocyte differentiation [ChIP-seq]

Project description:Waves of sumoylation support transcription dynamics during adipocyte differentiation [ChIP-seq]

| PRJNA703952 | ENA

Waves of sumoylation support transcription dynamics during adipocyte differentiation

Project description:Tight control of gene expression networks involved in adipose tissue formation and plasticity is required to adapt to energy needs and environmental cues. However, little is known about the mechanisms that orchestrate the dramatic transcriptional changes leading to adipocyte differentiation. We investigated the regulation of nascent transcription by SUMO during adipocyte differentiation using SLAMseq and ChIPseq. We discovered that SUMO has a dual function in differentiation; it supports the initial downregulation of pre-adipocyte-specific genes, while it promotes the establishment of the mature adipocyte transcriptional program. By characterizing SUMOylome dynamics in differentiating adipocytes by mass spectrometry, we found that SUMOylation of specific transcription factors like PPARG/RXR and chromatin modifiers promotes the transcription of adipogenic genes. Our data demonstrate that the sumoylation pathway helps coordinates the rewiring of transcriptional networks required for formation of functional adipocytes.

2022-02-18 | PXD024144 | Pride

Waves of sumoylation support transcription dynamics during adipocyte differentiation [ChIP-seq]

Project description:Tight control of gene expression networks involved in adipose tissue formation and plasticity is required to adapt to energy needs and environmental cues. However, little is known about the mechanisms that orchestrate the dramatic transcriptional changes leading to adipocyte differentiation. We investigated the regulation of nascent transcription by SUMO during adipocyte differentiation using SLAMseq and ChIPseq. We discovered that SUMO has a dual function in differentiation; it supports the initial downregulation of pre-adipocyte-specific genes, while it promotes the establishment of the mature adipocyte transcriptional program. By characterizing sumoylome dynamics in differentiating adipocytes by mass spectrometry we found that SUMOylation of specific transcription factors like PPAR/RXR and chromatin modifiers promotes the transcription of adipogenic genes. Our data demonstrate that the sumoylation pathway helps coordinates the rewiring of transcriptional networks required for formation of functional adipocytes.

2022-01-06 | GSE167220 | GEO

Waves of sumoylation support transcription dynamics during adipocyte differentiation.

Project description:Tight control of gene expression networks required for adipose tissue formation and plasticity is essential for adaptation to energy needs and environmental cues. However, the mechanisms that orchestrate the global and dramatic transcriptional changes leading to adipocyte differentiation remain to be fully unraveled. We investigated the regulation of nascent transcription by the sumoylation pathway during adipocyte differentiation using SLAMseq and ChIPseq. We discovered that the sumoylation pathway has a dual function in differentiation; it supports the initial downregulation of pre-adipocyte-specific genes, while it promotes the establishment of the mature adipocyte transcriptional program. By characterizing endogenous sumoylome dynamics in differentiating adipocytes by mass spectrometry, we found that sumoylation of specific transcription factors like PPARγ/RXR and their co-factors are associated with the transcription of adipogenic genes. Finally, using RXR as a model, we found that sumoylation may regulate adipogenic transcription by supporting the chromatin occurrence of transcription factors. Our data demonstrate that the sumoylation pathway supports the rewiring of transcriptional networks required for formation of functional adipocytes. This study also provides the scientists in the field of cellular differentiation and development with an in-depth resource of the dynamics of the SUMO-chromatin landscape, SUMO-regulated transcription and endogenous sumoylation sites during adipocyte differentiation.

| S-EPMC8860575 | biostudies-literature

Waves of sumoylation support transcription dynamics during adipocyte differentiation

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-01-06 | GSE167222 | GEO

TRIM28-Mediated SUMOylation of G3BP1/2 Regulates Stress Granule Dynamics

Project description:Stress granules (SGs) are stress-induced membraneless organelles whose dynamics are tightly regulated by protein interactions and modifications. However, whether SUMOylation directly targets SG core proteins G3BP1/2 and which ligase is involved remains unclear. Capturing these key events while preserving SG integrity is challenging due to their transient and membraneless nature. To address this, we introduce a low-concentration formaldehyde crosslinking (lcFAX) method that stabilizes membraneless organelles, including G3BP1 SGs and TDP-43 nuclear bodies, enabling enhanced proteome identification. Importantly, we identify TRIM28 as a previously undefined SG-associated protein and reveal that TRIM28 SUMOylates G3BP1 at K287 and G3BP2 at K281, establishing a critical mechanism regulating SG dynamics that ultimately impacts cellular ROS and apoptosis. lcFAX-Seq also provides insights into the RNA composition of SGs. Altogether, lcFAX-MS uncovers the critical role of TRIM28-mediated SUMOylation in modulating SG dynamics, establishing lcFAX as a powerful tool for analyzing membraneless organelles and the underlying regulatory mechanisms.

2025-09-24 | GSE286197 | GEO

Gingolic acid, a sumoylation inhibitor, promotes pre-adipogenesis but suppresses adipocyte maturation

Project description:Gingolic acid, a sumoylation inhibitor, promotes pre-adipogenesis but suppresses adipocyte maturation

| PRJNA414120 | ENA

TRIM28-Mediated SUMOylation of G3BP1/2 Regulates Stress Granule Dynamics

Project description:TRIM28-Mediated SUMOylation of G3BP1/2 Regulates Stress Granule Dynamics

| PRJNA1208106 | ENA

Site-specific SUMOylation proteomics in Arabidopsis

Project description:Identifying SUMOylation target sites in plants poses a significant challenge due to their relatively low abundance and high dynamics. Through this lysine-null SUMO1 method and the optimized two-step purification procedure, we successfully identified over 2,000 SUMOylation sites from 1,300 putative SUMO acceptors, significantly deepening the current SUMOylome in plants. Identification of thousands of SUMOylation sites offers a unique opportunity to comprehend the biological function and site specificity of SUMOylation at the proteome level.

2024-09-12 | PXD047170 | JPOST Repository

Site-specific SUMOylation proteomics in Arabidopsis

2024-09-12 | PXD047242 | JPOST Repository