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Prunus persica

ABSTRACT: Prunus persica MethylC-Seq segregating for mealiness

PROVIDER: PRJNA705001 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR13790801_1.fastq.gz	Fastqsanger.gz
	SRR13790801_2.fastq.gz	Fastqsanger.gz
	SRR13790802_1.fastq.gz	Fastqsanger.gz
	SRR13790802_2.fastq.gz	Fastqsanger.gz
	SRR13790803_1.fastq.gz	Fastqsanger.gz

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Project description:We charactarize the regulated miRNAs of Prunus persica to preventive treatment of PpPep2 1 µM endogenous peptide. The peptide treatment was applied to leaves and we sequenced the miRNAs after 1 and 24 h using untreated plants as control. miRNA profiles were generated by deep sequencing, in triplicate, using Illumina HiSeq 2500. Differential expression analysis of annotated pre_miRNA gene biotypes was performed with R package DESeq2 v1.26.0, considering significant epression values below FDR <0.05. We obtained 117,267,647 raw reads and we identified a total of 33 differential expressed miRNAs (DEM) belonging to 18 miRNA families upon PpPep2 application, accounting for 15% of the P. persica annotated miRNAs. miRNA regulation occurs principally one day after peptide treatment. The predicted mRNA targets and function of orthologous miRNAs are compatible with a regulation of PTI-related processes. Together with the transcriptomic response of P. persica to PpPep2 described at our previous RNA-Seq (Foix et al 2021), this suggests that miRNAs would have an active role in PTI regulation at these stages.

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