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Transcriptome analysis of the microplast derived from MCF7 cells treated with macrophage conditioned medium

ABSTRACT: Transcriptome analysis of the microplast derived from MCF7 cells treated with macrophage conditioned medium

PROVIDER: PRJNA705460 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13805993_1.fastq.gz	Fastqsanger.gz
	SRR13805993_2.fastq.gz	Fastqsanger.gz
	SRR13805994_1.fastq.gz	Fastqsanger.gz
	SRR13805994_2.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of the microplast derived from MCF7 cells treated with macrophage conditioned medium

Project description:Purpose: Next-generation sequencing was carried out to characterize transcripts landscape of the Microplast derived from the MCF7 cells treated with macrophage conditioned medium. Methods: To stimulate microplasts formation, MCF-7 cells were cultured in 50% macrophage conditioned medium and 50% DMEM growth medium for 48 h. Cells and microplasts were harvested and resuspended in ice cold PBS. A low-speed differential centrifugation protocol was used to isolate microplasts. Total RNA was extracted from two biological replicates of microplasts enriched samples using RNeasy mini kit (Qiagen, Hilden, Germany). RNA concentration and integrity were determined with Nanodrop 1000 (Nanodrop, Wilmington, DE,USA) spectrophotometry. Libraries were prepared using the TruSeq RNA sample prep kit V2 as per the manufacturer’s protocol (Illumina, San Diego, USA). Conclusions: This study provide the first transcriptional profile of the microplast derived from the MCF7 cells.

2021-05-18 | GSE167884 | GEO

Analysis by Whole Transcriptome Sequencing of the Effects of LLC1 conditioned medium, LLC1 conditioned plus Calcitriol, non-conditioned medium, and non-conditioned medium plus Calcitriol on mRNA Expression in Primary Human Skeletal Muscle Cells

Project description:Primary human skeletal muscle cells (Lonza) were treated with LLC1 conditioned medium, LLC1 conditioned medium plus Calcitriol, LLC1 non-conditioned medium or LLC1 non-conditioned medium plus Calcitriol for a period of 24 hours prior to isolation of RNA.

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MCF7 breast cancer cells treated with mesenchymal stem cell-derived exosomes

Project description:Human mesenchymal stem cell (MSC)-conditioned medium (CM) was previously reported to affect the biology of tumor cells; however, the precise mechanisms remain unclear. Here, we show that MSCs secreted 40- to 100-nm particles, which have the typical characteristics of exosomes, and these MSC-derived exosomes promoted migration of the breast cancer cell line MCF7. To further investigate the effect of MSC-exosomes on MCF7, we analyzed the gene expression profiles of MCF7 treated with or without MSC-exosomes for 24 h.

2013-05-20 | GSE46950 | GEO

Gene expression of breast cancer cell lines treated with conditioned medium derived from NAF and CAF

Project description:We treated nine lines of breast cancer cells with conditioned medium derived from NAF and CAF to study the paracrine interaction beetween stroma and tumor. Gene expression profiles for breast cancer cell lines are reported after 72h of treatment with conditioned medium derived from NAF and CAF or with control media.

2016-11-01 | GSE70884 | GEO

RNA-seq data of human T cells treated with decidual cell conditioned medium

Project description:RNA-seq data of Naïve T cells treated with decidual cell conditioned medium. Naïve T cells were differentiated into Th17 cells by IL6, TGFB1, and IL23 stimulation, in the presence of either unconditioned (control) or conditioned medium from uterine decidual stromal cells

2020-07-01 | GSE120380 | GEO

RNA from mouse lung conditioned medium

Project description:RNA from mouse lung conditioned medium

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LC-MSMS of microplasts derived from macrophage conditioned medium treated breast adenocarcinoma MCF-7 cells. Pooja Kamal Melwani, M.Sc., Nishad S, PhD, Badri Narain Pandey, PhD, Radiation Signalling and Cancer Biology Section, Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400 085, India

Project description:Paracrine signals relayed between heterogenous cell types through small and large extracellular vesicles promote cancer cell growth, invasion and metastasis. Microplasts, also called as cytoplasts or cytokineplasts are large extracellular vesicles (0.5-11µm diameter) originating from migratory cells. Microplasts are known to be associated with invasive phenotypes of cancer cells and promote metastasis. Treatment with macrophage conditioned medium induces significant shedding of microplasts from MCF-7 breast adenocarcinoma cells. To characterise microplasts and to study their potential role in intercellular communication in cancer, we isolated microplasts derived from macrophage conditioned medium treated MCF-7 cells and investigated their protein cargo through LC-MSMS.

2021-05-05 | PXD024293 | Pride

Protein Amount in Conditioned Medium of MenSCs

Project description:Protein Amount in Conditioned Medium of MenSCs

| PRJNA950089 | ENA

Microarray Analysis of Genes Regulated by Macrophage-Conditioned Media Treatment of Human Pre-Adipocytes

Project description:A major development in the study of obesity is the recognition that the condition is characterised by chronic mild inflammation. Within adipose tissue, this involves the infiltration of macrophages, as well as the direct inflammatory response of the adipocytes and pre-adipocytes. This study has used Agilent whole-genome microarrays to examine the effects of macrophage-conditioned medium on the global inflammatory response of human pre-adipocytes. Human pre-adipocytes (SGBS cells) were treated with macrophage (U937 cells) conditioned medium for 24 h. Control pre-adipocytes were treated with unconditioned medium (control) or RPMI-1640 media alone to control for differences in media used to culture the U937 cells. There were 5 biological replicates per group.

2011-12-21 | E-GEOD-27503 | biostudies-arrayexpress

RNA from mouse lung conditioned medium

Project description:RNA fragments were isolated from mouse lung conditioned medium and analyzed by a microarray This analysis surveys RNAs, probably complexed with RNA-binding protein, secreted in the culture media

2021-04-11 | GSE161219 | GEO

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