Project description:In order to study the effect of polyploidization on gene expression in the leaves of Eucalyptus urophylla, triploid obtained by sexual polyploidization and its diploid control were used as materials, and leaves at different growth stages of different ploidies were collected for transcriptome sequencing.
Project description:The expression pattern of transcripts involved in secondary development was determined in four genotypes of E. tereticornis using 8x60K Gene chip. The genome-wide expression data was used to develop a gene co-expression network for xylogenesis. Agilent one-color experiment, Organism: Eucalyptus,Custom Agilent Eucalyptus 8x60k Microarray Gene expression (AMADID: 59849 ) designed by Genotypic Technology Pvt.Ltd.
Project description:We present a label free proteome dataset of the vascular sap proteome of three commercially important Eucalyptus species (Eucalyptus camaldulensis, Eucalyptus grandis and Eucalyptus urophylla). Protein extraction from the vascular system was carried out using a pressure bomb, in solution digested and peptides were analyzed using a Q-Exactive instrument. Protein identification was carried out using stringent database searches and only in silico predicted extracellular proteins were considered as part of the sap proteome. The results here described can be used as a reference for the proteome sap analysis of Eucalyptus plants grown under different conditions.
Project description:Eucalyptus urophylla is a commercially important wood crop plantation species due to its rapid growth, biomass yield, and use as bioenergy feedstock. We characterized the genetic diversity and population structure of 332 E. urophylla individuals from 19 geographically defined E. urophylla populations with a reliability of 14,468 single nucleotide polymorphisms (SNPs). We compared the patterns of genetic variation among these 19 populations. High levels of genetic diversity were observed throughout the 19 E. urophylla populations based on genome-wide SNP data (HE=0.2677 to 0.3487). Analysis with STRUCTURE software, Principal component analysis (PCA) and a neighbor-joining (NJ) tree indicated that E. urophylla populations could be divided into three groups, and moderate and weak population structure was observed with pairwise genetic differentiation (FST) values ranging from −0.09 to 0.074. The low genetic diversity and shallow genetic differentiation found within the 19 populations may be a consequence of their pollination system and seed dispersal mechanism. In addition, 55 core germplasms of E. urophylla were constructed according to the genetic marker data. The genome-wide SNPs we identified will provide a valuable resource for further genetic improvement and effective use of the germplasm resources.