Project description:Characterization of the transcriptomic responses of grafted tomato seedlings leaves after the root inoculations with the two beneficial microorganisms Paraburkholderia graminis and Azospirillum brasiliensis. Paraburkholderia graminis treatment led to a higher number of differentially expressed genes than Azospirillum brasiliensis, with a higher amount of up-regulated than down-regulated genes for both treatments. These DEGs were manly involved in response to oxidative stress, response to biotic and abiotic stress, water transport, regulation of transcription and hormones. Only few DEGs were shared among the two treatments, including genes involved in flowering time and in tolerance against abiotic stresses.
Project description:This study investigated the transcriptomic response of Paraburkholderia busanensis P39 during exposure to fungal biomass of Colletotrichum scovillei KC05. RNA-seq was used to compare P39 grown alone with P39 exposed to fungal biomass, with three biological replicates per condition. The study aimed to identify transcriptional changes associated with fungal-biomass adaptation, nutrient scavenging, cell-envelope remodeling, regulatory responses, and interface-associated functions. Reads were aligned to the Paraburkholderia busanensis P39 genome represented by GenBank accessions CP058248 and CP058249.
Project description:Puccinia graminis f.sp. tritici (Pgt), the causal agent of stem rust disease in wheat, is one of the most destructive pathogens and can cause severe yield losses. Here, we utilize Hi-C sequencing technology to scaffold and phase the haplotypes for the genome assembly of a US Pgt isolate 99KS76A-1.