Project description:This study examines the proteomic responses in tissues of etiolated Sorghum bicolor BTx623 seedlings to salt stress (100 mM NaCl). The aim of the experiment is to investigate the induction and posssible role of Membrane Steroid Binding Protein 1 to salt tolerance in Sorghum.
Project description:This study used with RNA-Seq to examine the tissue specific expression data within sorghum plants for improving the Sorghum bicolor gene annotation. We examined the RNA from tissues (spikelet, seed and stem) in Sorghum bicolor (BTx623).Total RNAs form each tissues were extracted using SDS/phenol method followed by LiCl purification
Project description:This study utilized next generation sequencing technology (RNA-Seq and BS-Seq) to examine the transcriptome and methylome of various tissues within sorghum plants with the ultimate goal of improving the Sorghum bicolor annotation We examined the mRNA of various Sorghum bicolor (BTx623) tissues (flowers, vegitative and floral meristems, embryos, roots and shoots) and bisulfite treated DNA from two root samples
Project description:Parallel Analysis of RNA Ends (PARE) sequencing reads were generated to validate putative microRNAs and identify cleavage sites in Sorghum bicolor and Setaria viridis.
Project description:This study utilized next generation sequencing technology (RNA-Seq and BS-Seq) to examine the transcriptome and methylome of various tissues within sorghum plants with the ultimate goal of improving the Sorghum bicolor annotation
Project description:Flowering pathways are accelerated for rapid production of flowers and seeds in response to drought in certain varieties of sorghum (Sorghum bicolor (L.) Moench). The objective of the present study was to identify potential drought responsive genes that affect flowering time in sorghum under drought stress. Sorghum germplasm accessions representing early, intermediate, and late flowering groups were selected, and drought stress was administered on 25-day old seedlings of the Drought-Stressed group (DS) by withdrawing water whilst the control group of plants were well-watered (WW). At anthesis, with the initiation of pollen shedding, flag leaf tissues were harvested, and total RNA was separately isolated from samples. Transcription profiles consisting of 60 base pairs, paired end reads from total RNA of each sample were explored using Illumina Genome Analyzer deep sequencing method. An average of 66,059,932 clean reads were mapped. Among 10,468 differentially expressed genes, a set of 126 genes was up-regulated, and a set of 61 genes was down-regulated in all comparisons. Pathway enrichment analysis revealed de novo purine biosynthesis and lipoate biosynthesis pathways and Wnt signaling pathway affecting differentially expressed sorghum genes in response to drought. Transcriptome level differences among early, intermediate and late flowering groups of sorghum under WW and DS conditions were efficiently explored in the present study using RNA sequence analysis tools. Candidate genes and pathways that might be used to improve drought tolerance in sorghum were identified. Findings of the present study would lead to new targets for enhancing drought stress tolerance in sorghum.
Project description:This experiment contains the subset of data corresponding to sorghum RNA-Seq data from experiment E-GEOD-50464 (http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-50464/), which goal is to examine the transcriptome of various Sorghum bicolor (BTx623) tissues: flowers, vegetative and floral meristems, embryos, roots and shoots. Thus, we expanded the existing transcriptome atlas for sorghum by conducting RNA-Seq analysis on meristematic tissues, florets, and embryos, and these data sets have been used to improve on the existing community structural annotations.
