Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome profiling of muscle from control, mdx and Sirt6 knockout on mdx background mice

ABSTRACT: Transcriptome profiling of muscle from control, mdx and Sirt6 knockout on mdx background mice

PROVIDER: PRJNA714834 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR13976841.fastq.gz	Fastqsanger.gz
	SRR13976842.fastq.gz	Fastqsanger.gz
	SRR13976843.fastq.gz	Fastqsanger.gz
	SRR13976844.fastq.gz	Fastqsanger.gz
	SRR13976845.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 9

Similar Datasets

Transcriptome profiling of muscle from control, mdx and Sirt6 knockout on mdx background mice

Project description:In this study, Pax7-Cre mediated inactivation of Sirt6 in mdx mice resulted in profound improvement of the mdx phenotype at the functional level. To study the underlying molecular mechanisms we performed RNA-seq of muscles from control, mdx and Sirt6mKO/mdx mice.

2022-05-12 | GSE168984 | GEO

Transcriptome profiling of muscle stem cells from control, mdx and Sirt6 knockout on mdx background mice

Project description:Transcriptome profiling of muscle stem cells from control, mdx and Sirt6 knockout on mdx background mice

| PRJNA714832 | ENA

Transcriptome profiling of muscle stem cells from control, mdx and Sirt6 knockout on mdx background mice

2022-05-12 | GSE168983 | GEO

Inactivation of Sirt6 ameliorates muscular dystrophy in mdx mice by releasing suppression of utrophin expression

Project description:In this study, Pax7-Cre mediated inactivation of Sirt6 in mdx mice resulted in profound improvement of the mdx phenotype at the functional level. To study the underlying molecular mechanisms and identify specific Sirt6 targets irrespectively to mdx mutation, we performed RNA-seq of freshly isolated muscle stem cells from control and Sirt6mKO mice.

2022-05-12 | GSE199487 | GEO

Effects of Pten knockout in dystrophic skeletal muscles of mdx mouse

Project description:Analysis of skeletal muscles with specific knockout (KO) of Phosphatase and tensin homolog (Pten) gene in an animal model of DMD (mdx mice). Pten knockout alleviates myofiber degeneration and restores muscle function in mdx mice.

2020-10-06 | GSE150220 | GEO

iTRAQ Protemics of mdx vs. mdx/Actg1-TG mouse skeletal muscle

Project description:Duchenne muscular dystrophy (DMD) is an X-linked recessive disease caused by deleterious mutations in the DMD gene, rendering non-functional forms or complete absence of the protein dystrophin. Eccentric contraction-induced force loss is the most robust and reproducible phenotype of dystrophin-deficient skeletal muscle, yet the molecular mechanisms underlying force loss remain obscure. To this end, we utilized the mdx mouse model of DMD, which displays extreme sensitivity to eccentric contractions. An existing mouse line from our lab that overexpresses cytoplasmic gamma-actin specifically in skeletal muscle (mdx/Actg1-TG) was shown to significantly protect mdx muscle against contraction-induced force loss. To understand the mechanism behind this protection, we performed iTRAQ proteomics on mdx/Actg1-TG tibialis anterior (TA) muscle versus non-transgenic littermate controls to identify differentially-expressed proteins that may afford protection upon gamma-actin overexpression.

2018-10-22 | PXD009680 | Pride

Split intein mdx skeletal muscle proteomics

Project description:Duchenne muscular dystrophy is a rare and lethal neuromuscular disease caused by loss-of-function mutations in the dystrophin protein that provides structural integrity to striated muscle fibers. Mice with loss-of-function mutations for the Dmd gene encoding dystrophin (mdx-4cv) were treated with microdystrophin or split-intein AAV constructs to restore various-length dystrophin isoforms to the skeletal muscle compartment. The aim of this study was to compare protein expression profiles between healthy (WT), mdx-4cv, and AAV-treated mdx-4cv gastrocnemius skeletal muscle. We employed an isobaric labeling TMT multiplex discovery proteomics approach to describe and compare proteomic profiles across experimental groups.

2025-11-06 | PXD062324 | Pride

mRNA expression in regenerated mdx mouse skeletal muscle

Project description:Despite over 3,000 articles published on dystrophin in the last 15 years, the reasons underlying the progression of the human disease, differential muscle involvement, and disparate phenotypes in different species are not understood. The present experiment employed a screen of 12,488 mRNAs in 16-wk-old mouse mdx muscle at a time when the skeletal muscle is avoiding severe dystrophic pathophysiology, despite the absence of a functional dystrophin protein. A number of transcripts whose levels differed between the mdx and human Duchenne muscular dystrophy were noted. A fourfold decrease in myostatin mRNA in the mdx muscle was noted. Differential upregulation of actin-related protein 2/3 (subunit 4), beta-thymosin, calponin, mast cell chymase, and guanidinoacetate methyltransferase mRNA in the more benign mdx was also observed. Transcripts for oxidative and glycolytic enzymes in mdx muscle were not downregulated. These discrepancies could provide candidates for salvage pathways that maintain skeletal muscle integrity in the absence of a functional dystrophin protein in mdx skeletal muscle. Keywords: other

2003-07-16 | GSE466 | GEO

Leg muscle mdx and control

Project description:Leg muscle mdx and control: 7,14,23 day Keywords: other

2004-02-05 | GSE1018 | GEO

Effect of Sirt6 deficiency on transcriptome in skeletal muscle of mice

Project description:Expanding the exercise capacity is an emerging strategy to combat metabolic diseases. Skeletal muscle fiber type determination is critical for the exercise performance, but the regulatory basis is largely unknown. Here, we report that Sirt6 has a role in regulating myofiber configuration toward oxidative type and that Sirt6 activator can be an exercise mimetic. To elucidate molecular mechanisms, we performed RNA-sequencing analysis using WT and skeletal muscle-specific Sirt6 KO mice. Transcriptomic analysis enabled us to discover major signaling nodes altered by Sirt6 deficiency in close association with mitochondrial function and muscle phenotypes.

2022-02-25 | GSE186105 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data