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The effect of the modulation of L2-hydroxyglutarate content in pachytene-diplotene cells of male mice

ABSTRACT: The effect of the modulation of L2-hydroxyglutarate content in pachytene-diplotene cells of male mice

PROVIDER: PRJNA714901 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13977829.fastq.gz	Fastqsanger.gz
	SRR13977830.fastq.gz	Fastqsanger.gz
	SRR13977831.fastq.gz	Fastqsanger.gz
	SRR13977832.fastq.gz	Fastqsanger.gz
	SRR13977833.fastq.gz	Fastqsanger.gz

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The effect of the modulation of L2-hydroxyglutarate content in pachytene-diplotene cells of male mice

Project description:We found that testis of male mice contain high concentrations of L2-hydroxyglutarate (L2HG) specifically in germ cells at pachytene/ diplotene (PD) stage of spermatogenesis. In order to elucidate the function of 2HG we decreased its level by incubating PD cells with 24 mM oxamate and to rescue the putative phenotype by incubation with 24 mM oxamate together with 0.3 mM cell permeable L2HG.

2024-03-25 | GSE169015 | GEO

Small RNA profiling of TUT4 and TUT7-deficient mouse pachytene-diplotene cells.

Project description:To study the impact of TUT4 and TUT7 on miRNA and piRNA biology, Tut4 and Tut7 were conditionally deleted in differentiating spermatogonia. Total RNA samples from TUT4 and TUT7-deficient pachytene-diplotene cells were used to generate small RNAs libraries.

2019-01-14 | E-MTAB-7065 | biostudies-arrayexpress

The effect of 2HG level on transcriptome at different stages of male mouse meiosis

Project description:We found that L2-hydroxyglutarate (2HG) level is 15 times higher in pachytene-diplotene (PD) stage as compared to leptotene-zygotene (LZ) stage. Since 2HG shows epigenetic effects we asked if the addition of 2HG to LZ cells or the decrease of 2HG level in PD cells will cause changes in gene expression.

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Characterization of ZFP541's occupency sites on genome in pachytene and diplotene spermatocytes

Project description:The DSB-machinery, which induces the programmed DNA double-strand breaks (DSBs) in leptotene and zygotene stages during meiosis, needs to be kept in silence after the initiation of pachytene stage to prevent the activation of DSB checkpoint that may lead to meiotic arrest or apoptosis of germ cells. However, the mechanisms underlying this repression remain largely unknown. Here, we report that ZFP541, a germ cell-specific zinc finger protein, is responsible for the suppression of DSBs formation at late pachytene. Lack of Zfp541 in mice leads to generation of DSBs in late pachytene spermatocytes by DSB formation related-proteins and causes male infertility due to meiotic failure. Plated-based scRNA-seq of Zfp541-/- spermatocytes revealed that ZFP541 negatively regulates many meiotic prophase genes, including genes for DSB formation and their upstream transcriptional regulators, in late pachytene spermatocytes. These results were confirmed by 10x single-cell RNA-seq data on spermatogenesis of Zfp541-/- testes, which suggested that Zfp541 is required for repressing the activation of pre-pachytene gene expression programs from early to late pachytene. ZFP541 ChIP-seq on pachytene and diplotene spermatocytes demonstrated that ZFP541 occupies the promoters of meiosis initiators (e.g., Meiosin and Rxra) and a subset of their downstream genes to repress their transcription, and thus prevent the reactivation of pre-pachytene gene expression programs in pachytene spermatocytes. Thus, our results not only revealed the role of ZFP541 in maintaining the repression of pre-pachytene transcriptional programs in pachytene spermatocytes but also provide new insight into the regulation of meiotic progression by timely turning off pre-pachytene genes.

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Small RNA profiling of TUT4 and TUT7-deficient mouse pachytene-diplotene cells.

Project description:Small RNA profiling of TUT4 and TUT7-deficient mouse pachytene-diplotene cells.

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Characterization of ZFP541's occupency sites on genome in pachytene and diplotene spermatocytes

Project description:Characterization of ZFP541's occupency sites on genome in pachytene and diplotene spermatocytes

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ALKBH5 regulates transcriptomic changes from leptene/zygotene to pachytene/diplotene stages in spermatocytes

Project description:ALKBH5 regulates transcriptomic changes from leptene/zygotene to pachytene/diplotene stages in spermatocytes

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Expression profiling of isolated populations of prepachytene spermatocytes, pachytene spermatocytes and spermatids of PWD and B6 males

2014-06-01 | GSE49444 | GEO

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Project description:To study the roles of the proteins TUT4, TUT7 and UPF2 in spermatogenesis, their genes were conditionally deleted in differentiating spermatogonia. Total RNA samples from TUT4 and TUT7-deficient or UPF2-deficient pachytene/diplotene cells were subjected to array profiling.

2019-01-14 | E-MTAB-7062 | biostudies-arrayexpress

Expression profiling of isolated populations of prepachytene spermatocytes, pachytene spermatocytes and spermatids of PWD and B6 males

Project description:Expression profiling of isolated populations of prepachytene spermatocytes (LP), pachytene spermatocytes (RP) and spermatids (ST) from PWD and B6 was performed to study the genome wide variation in gene expression between two mouse subspecies. To evaluate the transcriptional difference between B6 and PWD in during meiosis, we compared their transcriptomes in sorted populations of pre-pachytene primary spermatocytes (Leptonema, Zygotene and Pachytene), pachytene spermatocytes (Mid-late pachytene and diplotene) and spermatids. Populations of pre-pachytene spermatocytes, pachytene spermatocytes and spermatids were isolated from PWD and B6 mice. All populations were isolated in duplicates from two animals. Hence, RNA was prepared from 12 samples (= 2 strains x 3 tissues x 2 replicas) and was then subject to labeling and hybridization on microarray chips.

2014-06-01 | E-GEOD-49444 | biostudies-arrayexpress

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