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MapToCleave: high-throughput profiling of microRNA biogenesis in living cells

ABSTRACT: MapToCleave: high-throughput profiling of microRNA biogenesis in living cells

PROVIDER: PRJNA714912 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13978000.fastq.gz	Fastqsanger.gz
	SRR13978001.fastq.gz	Fastqsanger.gz
	SRR13978002.fastq.gz	Fastqsanger.gz
	SRR13978003.fastq.gz	Fastqsanger.gz
	SRR13978004.fastq.gz	Fastqsanger.gz

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MapToCleave: high-throughput profiling of microRNA biogenesis in living cells

Project description:We here introduce MapToCleave, a new method to simultaneously profile processing of thousands of distinct RNA structures in living cells. We find that miRNA precursors connected to a stable lower basal stem are more efficiently processed and also have higher expression in vivo in tissues from twenty animal species. We systematically compare the importance of known and novel sequence and structural features, and test miRNA precursors from ten animal and plant species in human cells. Last, we provide evidence that the known GHG motif better predicts processing when defined as a structure rather than sequence motif, consistent with recent cryo-EM studies. In summary, we apply a new screening assay in living cells to reveal the importance of lower basal stem stability for miRNA processing and in vivo expression.

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