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Exosomal-miRNA sequencing of optineurin gene knockdown BV2 cells and wild type BV2 cells

ABSTRACT: Exosomal-miRNA sequencing of optineurin gene knockdown BV2 cells and wild type BV2 cells

PROVIDER: PRJNA715477 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14001493.fastq.gz	Fastqsanger.gz
	SRR14001494.fastq.gz	Fastqsanger.gz
	SRR14001495.fastq.gz	Fastqsanger.gz
	SRR14001496.fastq.gz	Fastqsanger.gz
	SRR14001497.fastq.gz	Fastqsanger.gz

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Exosomal-miRNA sequencing of optineurin gene knockdown BV2 cells and wild type BV2 cells

Project description:To compare the miRNA profiles in exosomes derived from optineurin gene knockdown (Optn-KD)BV2s cells and wild type (WT) BV2 cells, BV2 cells were divided into two groups, Optn-KD group or WT group. We treated the Optn-KD group with Optn siRNA for 24 hours. Then, MiRNA profiles for each group were examined by Illumina HiSeq4000. We found differentiated miRNA profiles in the two groups. Compared with WT BV2 cell-derived exosomes, 4 miRNAs (miR-125b-5p, miR-351-5p, miR-505-5p, miR-novel-chr8_37700) were up-regulated and 2 miRNAs (miR-574-5p, miR-99b-5p) were down-regulated in Optn-KD BV2 cell-derived exosomes. This study provides a framework for characterizing the exosomal-miRNAs in Optn-KD BV2 cells.

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Optineurin Shapes Basal and LPS-Induced Transcriptomes in BV2 Microglia

Project description:The OPTN gene is linked to neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), which are characterized by chronic microglial activation. Optineurin regulates inflammatory signaling, autophagy, and trafficking, but its role in microglia is not well understood. We used bulk RNA sequencing to profile CRISPR-Cas9-mediated optineurin knockout (KO) and wild-type (WT) BV2 microglia under basal conditions and upon LPS stimulation. At baseline, optineurin KO altered approximately 7% of the transcriptome, with a notable downregulation of type I interferon and antiviral pathways. LPS stimulation in wild-type cells triggered a broad transcriptional shift (~35% of genes). This LPS-induced response was blunted in optineurin-deficient microglia, with ~16% of genes changed relative to the KO baseline. Furthermore, LPS-treated optineurin KO microglia notably diverged from LPS-treated wild-type cells, with ~26% differentially expressed genes. Our findings establish optineurin as a key regulator of the microglial transcriptome. Its loss weakens basal interferon-mediated immune surveillance and decouples the canonical inflammatory response from cell cycle arrest upon activation.

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Optineurin Shapes Basal and LPS-Induced Transcriptomes in BV2 Microglia

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siRNA profiling of human cells knocked down for optineurin

Project description:This study aimed to identify genes that are linked with optineurin expression using a combined siRNA-microarray approach Experiment Overall Design: Samples a01, a02 and a03 were treated with siRNA specific for optineurin mRNA Experiment Overall Design: Samples b01, b02 and b03 were treated with water (mock experiment)

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