Role of the major determinant of polar flagellation FlhG in the endoflagella-containing spirochete Leptospira
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ABSTRACT:
PROVIDER: PRJNA719355 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR14134769.fastq.gz | Fastqsanger.gz | |||
| SRR14134770.fastq.gz | Fastqsanger.gz | |||
| SRR14134771.fastq.gz | Fastqsanger.gz | |||
| SRR14134772.fastq.gz | Fastqsanger.gz | |||
| SRR14134773.fastq.gz | Fastqsanger.gz |
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Project description:In this project, we investigated whether diverse spirochete species contain lysinoalanine cross-linkages between conserved residues in their flagellar hook proteins (FlgE). We examined the following species: Treponema denticola¸ Borreliella burgdorferi, Treponema phagedenis, Treponema pallidum, Brachyspira hyodysenteriae, Leptospira interrogans. For each bacterial species, we examined a variety of different sample types, including recombinantly produced FlgE, polyhooks purified from flik knock-out strains, and wild-type periplasmic filaments. Overall, our data suggests that lysinoalanine crosslinking in a conserved FlgE post-translational modification in spirochetes and is required for the unique motility of these organisms. For more information, please see the following manuscript: https://doi.org/10.1101/2023.06.13.544825.
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