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Locational memory of macrovessel vascular cells is transcriptionally imprinted

ABSTRACT: Locational memory of macrovessel vascular cells is transcriptionally imprinted

PROVIDER: PRJNA719562 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14140203.fastq.gz	Fastqsanger.gz
	SRR14140204.fastq.gz	Fastqsanger.gz
	SRR14140205.fastq.gz	Fastqsanger.gz
	SRR14140206.fastq.gz	Fastqsanger.gz
	SRR14140207.fastq.gz	Fastqsanger.gz

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Locational memory of macrovessel vascular cells is transcriptionally imprinted

Project description:The locational predisposition of vascular pathologies illustrates the need for a better insight into vascular heterogeneity. To investigate the transcriptomic basis of angiodiversity, we isolated and analyzed transcriptomes from endothelial cells and vascular smooth muscle cells from nine different adult canine macrovessels: the aorta, coronary artery, vena cava, portal vein, femoral artery, femoral vein, saphenous vein, pulmonary vein, and pulmonary artery. We identified both reported and novel expression patterns defining specialized adult blood vessels. Our findings also show that adult vascular cells in culture express a remarkably high number of transcription factors crucial to organ development in the embryo. The persistent expression of these genes in culture indicates that these genes are not regulated by the flow or surrounding cell types but are rather fixed in the molecular memory. Therefore, our findings prompt the re-thinking of the extrapolation of results from single-origin endothelial cell systems.

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Locational memory of macrovessel vascular cells is transcriptionally imprinted.

Project description:Vascular pathologies show locational predisposition throughout the body; further insights into the transcriptomics basis of this vascular heterogeneity are needed. We analyzed transcriptomes from cultured endothelial cells and vascular smooth muscle cells from nine adult canine macrovessels: the aorta, coronary artery, vena cava, portal vein, femoral artery, femoral vein, saphenous vein, pulmonary vein, and pulmonary artery. We observed that organ-specific expression patterns persist in vitro, indicating that these genes are not regulated by blood flow or surrounding cell types but are likely fixed in the epigenetic memory. We further demonstrated the preserved location-specific expression of GATA4 protein in cultured cells and in the primary adult vessel. On a functional level, arterial and venous endothelial cells differed in vascular network morphology as the arterial networks maintained a higher complexity. Our findings prompt the rethinking of the extrapolation of results from single-origin endothelial cell systems.

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