Project description:The Oreochromis niloticus was infected with S.agalactiae strain YM001, than collected the liver,spleen,brain,intestine and kidney tissue for proteome
Project description:Evolutionary alterations to cis-regulatory sequences are likely to cause adaptive phenotypic complexity, through orchestrating changes in cellular proliferation, identity and communication. For non-model organisms with adaptive key-innovations, patterns of regulatory evolution have been predominantly limited to targeted sequence-based analyses. Chromatin-immunoprecipitation with high-throughput sequencing (ChIP-seq) is a technology that has only been used in genetic model systems and is a powerful experimental tool to screen for active cis-regulatory elements. Here, we show that it can also be used in ecological model systems and permits genome-wide functional exploration of cis-regulatory elements. As a proof of concept, we use ChIP-seq technology in adult fin tissue of the cichlid fish Oreochromis niloticus to map active promoter elements, as indicated by occupancy of trimethylated Histone H3 Lysine 4 (H3K4me3). The fact that cichlids are one of the most phenotypically diverse and species-rich families of vertebrates could make them a perfect model system for the further in-depth analysis of the evolution of transcriptional regulation. examination of H3K4me3 in adult fin tissue of the Nile tilapia (Oreochromis niloticus)
Project description:This study examines genomic copy-number variation among African cichlids spanning multiple tribes and radiations. We map CNVs and hotspots throughout the Oreochromis niloticus reference genome, categorize gene ontology enrichment within CNV regions, and compare results with sequence-based cichlid phylogenies.
Project description:A quantitative proteomics approach was used to investigate in Oreochromis niloticus cell line across two passages P10, and P20. A cell line developed from the muscle tissue of O. niloticus (ONM) was utilized to understand the proteome in-depth. Comparative proteome analysis was performed for ONM muscle cells between two passages (10 and 20) using label free quantitative proteomics. High-resolution mass spectrometry was used for data using label-free quantification. Data were further analysed using a statistical tool across passages of rohu muscle cell line. Proteomic analysis across two passages (P10 and P20) revealed pathways involved in myogenesis were striated muscle contraction, muscle contraction, insulin pathway, regulation of actin cytoskeleton, hedgehog on state, VEGFA-VEGFR2 and signalling by WNT. The cell line generated can have wide range of application in cultivated meat as well as in in vitro application.