Project description:The goal of this study was to investigate differential regulation of miRNA expression in the cervical tissue of women with low-grade cervical intraepithelial neoplasia (LGCIN, or low grade cervical dysplasia). We compared the miRNA expression profiles of women with LGCIN that progressed to high-grade CIN to the miRNA expression profiles of women with LGCIN that naturally resolved without medical intervention.
Project description:To investigate lncRNA-miRNA-mRNA network interactions with clinicopathological outcomes in gastric cancer, microarray analysis was obtained by microRNA profiling from the patient samples. The potential target genes were then predicted by miRmap, targetscan, and miRWalk2.0. The candidate lncRNAs were collected by the LncRNA2target and TANRIC databases. The expression levels of candidate lncRNAs, miRNAs, and mRNAs were measured in tumor and normal samples using qRT-PCR and ELIZA techniques and compared with the clinicopathological outcomes. We then performed gene expression profiling analysis using data obtained from RNA-seq of two tissue samples of gastric cancer patients.
Project description:Clear cell renal cell carcinomas (ccRCC) are characterized by arm-wide chromosomal alterations. Loss at 14q is associated with disease aggressiveness in ccRCC, which responds poorly to chemotherapeutics. The 14q locus contains one of the largest miRNA clusters in the human genome; however, little is known about the contribution of these miRNAs to ccRCC pathogenesis. In this regard, we investigated the expression pattern of selected miRNAs at the 14q32 locus in TCGA kidney tumors and in ccRCC cell lines. We validated that the miRNA cluster is downregulated in ccRCC (and cell lines) as well as in papillary kidney tumors relative to normal kidney tissues and primary renal proximal tubule epithelial (RPTEC) cells. We demonstrated that agents modulating expression of DNMT1 (e.g., 5-Aza-deoxycytidine) could modulate miRNA expression in ccRCC cell lines. Lysophosphatidic acid (LPA, a Lysophospholipid mediator elevated in ccRCC) not only increased labile iron content but also modulated expression of 14q32 miRNAs. Through an overexpression approach targeting a subset of 14q32 miRNAs (specifically at subcluster A: miR-431, miR-432, miR-127, and miR-433) in 769-P cells, we uncovered changes in cellular viability and claudin-1, a tight junction marker. A global proteomic approach was implemented using these miRNA overexpressing cell lines which uncovered ATXN2 as a highly downregulated target, which has a role in chronic kidney disease pathogenesis. Collectively, these findings support a contribution of miRNAs at 14q32 in ccRCC pathogenesis.
Project description:To investigate the differential dynamic plasma miRNA profiles during the peri-implantation period in patients with different reproductive outcomes following embryo transfer. 10 negative pregnancy (NP) patients, 10 biochemical pregnancy loss (BPL) patients, and 10 clinical pregnancy (CP) patients were enrolled for miRNA-seq in this study. Peripheral blood samples were collected at three time-points (ET0, ET11, and ET14) from BPL patients and CP patients, and samples were collected at two time-points (ET0 and ET11) from NP patients. Small RNA libraries were constructed using peripheral plasma RNA. miRNA-seq was used to investigate the differential dynamic peripheral miRNA changes between the three groups during the peri-implantation period.
