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PD-L1 alteration in lung cancer affects tumorigenicity lung tumors

ABSTRACT: PD-L1 alteration in lung cancer affects tumorigenicity lung tumors

PROVIDER: PRJNA720411 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14165601.fastq.gz	Fastqsanger.gz
	SRR14165602.fastq.gz	Fastqsanger.gz
	SRR14165603.fastq.gz	Fastqsanger.gz
	SRR14165604.fastq.gz	Fastqsanger.gz
	SRR14165605.fastq.gz	Fastqsanger.gz

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PD-L1 alteration in lung cancer affects tumorigenicity lung tumors

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Project description:Lung cancer is a major global health problem, as it is the leading cause of cancer- related deaths worldwide. Non-small-cell lung cancer (NSCLC), the most common form, is a heterogeneous disease with adenocarcinoma and squamous cell carcinoma being the predominant subtypes. Immune-inhibiting interaction of Programmed cell death-ligand 1 (PD-L1) with programmed cell death-protein 1 (PD-1) causes checkpoint mediated immune evasion and is, accordingly, an important therapeutic target in cancer. In NSCLC, improved understanding of PD-1/PD-L1 checkpoint blockade-responsive biology is warranted. We aimed to identify the landscape of immune cell infiltration in primary lung adeno- carcinoma (LUAD) in the context of tumor PD-L1 expression and the extent of immune infiltration (“hot” vs. “cold” phenotype). Therefore, the study comprises LUAD cases (n=138) with “hot” and “cold” tumor immune phenotype and positive and negative tumor PD-L1 expression, respectively. Tumor samples were immunohistochemically analyzed for expression of PD-L1, CD4 and CD8 and further analyzed on transcriptomic level by Nanostring nCouter Pan Cancer Immune Profiling Panel. We detected significantly differentially expressed genes associated with PD-L1 positive and “hot” versus PD-L1 negative and “cold” phenotype. The presented study illustrates novel aspects of PD-L1 regulation, with potential biological relevance, as well as relevance for immunotherapy response stratification.

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