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Gene regulation by m6A demethylase ALKBH5 in U2OS osteosarcoma

ABSTRACT: Gene regulation by m6A demethylase ALKBH5 in U2OS osteosarcoma

PROVIDER: PRJNA720547 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14181706.fastq.gz	Fastqsanger.gz
	SRR14181707.fastq.gz	Fastqsanger.gz
	SRR14181708.fastq.gz	Fastqsanger.gz
	SRR14181709.fastq.gz	Fastqsanger.gz
	SRR14181710.fastq.gz	Fastqsanger.gz

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Gene regulation by m6A demethylase ALKBH5 in U2OS osteosarcoma

Project description:To identify target genes regulated by ALKBH5 in osteosarcoma, we silenced the expression of ALKBH5 in osteosarcoma cell line-U2OS and tested its effect on U2OS transcriptome.

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Gene regulation by m6A demethylase ALKBH5 in osteosarcoma

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Project description:N6-methyladenosine (m6A) is the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Here we report ALKBH5 as a new mammalian demethylase that oxidatively removes the m6A modification in mRNA in vitro and inside cells. This demethylation activity of ALKBH5 significantly affects mRNA export and RNA metabolism as well as the assembly of mRNA processing factors in nuclear speckles. Alkbh5-deficient male mice are characterized by impaired fertility resulting from apoptosis that affects meiotic metaphase-stage spermatocytes. In accordance with this defect, we have identified in mouse testes 1552 differentially expressed genes which cover broad functional categories and include spermatogenesis-related mRNAs involved in the p53 functional interaction network. We show that Alkbh5-deficiency impacts the expression levels of some of these mRNAs, supporting the observed phenotype. The discovery of this new RNA demethylase strongly suggests that the reversible m6A modification plays fundamental and broad functions in mammalian cells. RNA-seq in two cell types

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Gene regulation by m6A demethylase ALKBH5 in osteosarcoma [RNA-seq]

Project description:Gene regulation by m6A demethylase ALKBH5 in osteosarcoma [RNA-seq]

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Gene regulation by m6A demethylase ALKBH5 in osteosarcoma [RNA-seq]

Project description:To identify target genes regulated by ALKBH5 in osteosarcoma, we silenced the expression of ALKBH5 in osteosarcoma cell line-143B and tested its effect on 143B transcriptome.

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Project description:ALKBH5 is the RNA N(6)-methyladenosine (m6A) demethylase. To under sthand the function and mechnism of ALKBH5 in human acute myeloid leukemia, we compared the m6A profiling in wild-type, ALKBH5-knock-down, and ALKBH5 rescue THP1 cells.

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