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CITE-seq of 6 patients treated with the Pfizer BioNTech BNT162b2 mRNA vaccine

ABSTRACT: CITE-seq of 6 patients treated with the Pfizer BioNTech BNT162b2 mRNA vaccine

PROVIDER: PRJNA721461 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14219700.fastq.gz	Fastqsanger.gz
	SRR14219701.fastq.gz	Fastqsanger.gz
	SRR14219702.fastq.gz	Fastqsanger.gz
	SRR14219703.fastq.gz	Fastqsanger.gz
	SRR14219704.fastq.gz	Fastqsanger.gz

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CITE-seq of 6 patients treated with the Pfizer BioNTech BNT162b2 mRNA vaccine

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Project description:The experiment aims at characterizing the immune responses elicited by the BNT162b2 vaccine against SARS-CoV-2, initially administered in a two dose regimen (second dose after three weeks followinf the first dose) In particular the transcriptional landscape of circulating T and B lymphocytes has been profiled longitudinnaly by scRNA-seq coupleD with CITE-seq of 19 cell surface markers to better classify T cells subpopulations, LIBRA-seq to assess the Spike-specificity of BCRs and and V(D)J seq to also track T and B cell clones dynamics. Eeach sample was profiled before vaccination (T0), 21 days after the first dose (T1), 2 months after the first dose (1 month after the second dose) (T2). The immune responses were characterized using PBMC from 3 SARS-CoV-2 experienced donors (experiencing SARS-Cov-2 at least 4 months before the first vaccinatin) and 2 SARS-CoV-2 unexperienced donors.

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