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Transcriptional profiling of murine small intestine in conventional or SPF housing conditions

ABSTRACT: Transcriptional profiling of murine small intestine in conventional or SPF housing conditions

PROVIDER: PRJNA721667 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14229237.fastq.gz	Fastqsanger.gz
	SRR14229238.fastq.gz	Fastqsanger.gz
	SRR14229239.fastq.gz	Fastqsanger.gz
	SRR14229240.fastq.gz	Fastqsanger.gz
	SRR14229241.fastq.gz	Fastqsanger.gz

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Transcriptional profiling of murine small intestine in conventional or SPF housing conditions

Project description:We studied the influence of the microbiota on intestinal homeostasis and tumor development after loss of the intestinal tumor phenotype in Msh2-Lynch mice upon rederivation of the mouse line in an SPF facility. We generated RNA seq data from total RNA of small intestine (jejunum) derived from either conventional or SPF mice.

2022-02-08 | GSE171996 | GEO

Augmented murine hematopoietic stem and progenitor cell reconstruction through conventional co-housing

Project description:Background and methods: The effects of microbiota toward hematopoietic stem and progenitor cells (HSPCs) were very much complicated based on previous reports. In the current study, normal inbred C57BL6 (B6) mice from the Jackson Laboratory were maintained in the SPF environment for three weeks. Baseline complete blood counts were normal, and animals were then separated into two groups: 1) SPF mice stayed in the SPF facility, and 2) SPF mice being transferred to a conventional facility for co-housing (CVT). At one month of co-housing, we bled and euthanized animals, sorted lineage-CD117+ cells from bone marrow of SPF and CVT mice respectively, and performed single cell RNA sequencing on sorted hematopoietic stem and progenitor cells using 10XGenomics Chromium Single Cell 3' Solution and sequenced with Illumina HiSeq 3000 Systems. Results: We characterized heterogeneity of HSPCs, defined differential gene expression and performed pathway analysis. Proportion of LTHSC was lower and the proportion of GMP was higher from CVT mice than those from SPF mice. Pathway analyses of differentially-expressed genes in HSPCs we found down-regulation of genes related to immune responses and cell proliferation and activation, and up-regulation of genes associated with cellular metabolism, including protein, ribosome, and energy metabolic processes, in cells from CVT mice. Conclusion: Observations from our study demonstrated a positive effect in co-housed CVT mice that promotes HSPC reconstitution through modulation of molecular elements involving multiple signaling pathways.

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