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Transcriptomes of the developing larval ovaries of Drosophila melanogaster during terminal filament formation

ABSTRACT: Transcriptomes of the developing larval ovaries of Drosophila melanogaster during terminal filament formation

PROVIDER: PRJNA721702 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14231540.fastq.gz	Fastqsanger.gz
	SRR14231541.fastq.gz	Fastqsanger.gz
	SRR14231542.fastq.gz	Fastqsanger.gz
	SRR14231543.fastq.gz	Fastqsanger.gz
	SRR14231544.fastq.gz	Fastqsanger.gz

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Transcriptomes of the developing larval ovaries of Drosophila melanogaster during terminal filament formation

Project description:Insect ovaries are made up of tubular egg producing subunits called ovarioles, whose number largely determines the number of eggs that can be potentially laid. Ovariole number is directly determined by the number of cellular structures called terminal filaments, which are stacks of cells that assemble in the larval ovary. Elucidating the developmental and regulatory mechanisms of terminal filament formation is thus key to understanding the regulation of insect reproduction through ovariole number regulation. We systematically measured mRNA expression of all cells in the Drosophila larval ovary at the beginning, middle and end of terminal filament formation using RNA-seq. We also separated somatic and germ line cells during these stages and assessed their tissue-specific gene expression during larval ovary development.

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Project description:Adult insect ovaries are made up of egg production structures called ovarioles, whose number is directly linked to fecundity and fitness. Ovariole number is directly determined by the number of cell stacks called terminal filaments in the larval ovary, which in turn is determined by the number of terminal filament cells available to form terminal filaments. Elucidating the developmental and genetic regulation of terminal filament cell proliferation is thus key to understanding the regulation of adult ovariole number and fitness. Our previous work showed that the Hippo signaling pathway, including the kinase hippo and the transcription factor yorkie, regulate terminal filament cell number in Drosophila melanogaster. To identify the specific transcriptional responses of ovarian cells to Hippo signaling during ovary development, we systematically measured mRNA expression in isolated pools of somatic and germ line cells from the Drosophila larval ovary at the beginning, middle and end of terminal filament formation using RNA-seq. We performed these RNA-seq experiments in wild type, hippoRNAi and yorkieRNAi genetic backgrounds at each of these three developmental stages.

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