Project description:The atmosphere CO2 concentration keeps increasing every year. Use the Affymetrix poplar gene chip to confirm the expression changes in key genes in the triploid white poplar due to the influence of elevated CO2 concentrations. We used microarrays to detail the global programme of gene expression under normal and elevated CO2 concentrations. Gene expression of triploid white poplar ((P. tomentosa Ã? P. bolleanaï¼?Ã? P. tomentosa) leaves were investigated by using the Affymetrix poplar genome gene chip, after grown in controlled environment chambers under three different CO2 concentrations. Poplar leaves were subjected to normal CO2 concentrations (T0) and elevated CO2 concentrations (T1, 550 ppm and T2, 720 ppm) treatments three months.
Project description:We sequenced mRNA from the control and heat treatments leaves of Populus tomentosa using the Illumina HiSeq4000 platform to generate the transcriptome dynamics that may serve as a gene expression profile blueprint for different response patterns under control and heat stress in Populus tomentosa.
Project description:To dissect the gene regulatory networks operating during soybean embryo development, we identified the binding sites genome-wide for transcription factor GLYMA.04G093300 and GLYMA.06G314400 in cotyledon stage embryos using ChIP-seq.
Project description:Diet strongly shapes the host’s transcriptional response to P. tomentosa exposure, suggesting that nutritional status influences both parasite burden and the host’s capacity to mount an effective molecular response.