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CD127+CD94+ innate lymphoid cells expanded in Crohn’s disease patients highly express granulysin and perforin

ABSTRACT: CD127+CD94+ innate lymphoid cells expanded in Crohn’s disease patients highly express granulysin and perforin

PROVIDER: PRJNA726520 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14369826.fastq.gz	Fastqsanger.gz
	SRR14369827.fastq.gz	Fastqsanger.gz
	SRR14369828.fastq.gz	Fastqsanger.gz
	SRR14369829.fastq.gz	Fastqsanger.gz
	SRR14369830.fastq.gz	Fastqsanger.gz

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CD127+CD94+ innate lymphoid cells expanded in Crohn’s disease patients highly express granulysin and perforin

Project description:Controversy in the identity and distinction of helper ILC1s and NK cells exist due to overlapping markers and the use of different gating strategies by distinct groups. Recently we identified cytotoxic ILC3s characterized by expression of CD94. Here we analyzed the full spectrum of CD127+ ILCs and NK cells in intestinal lamina propria from healthy donors and Crohn’s disease patients and identified two populations of CD127+CD94+ ILCs, designated A and B that could be distinguished on expression of CD117, CD18 and cytotoxic molecules. Population B highly expressed granulysin, a cytotoxic molecule linked to bacterial lysis and/or chemotaxis of monocytes. Granulysin protein was amply secreted by population B cells upon stimulation with IL-15. Activation of population B in the presence of TGF-β strongly reduced the expression of cytotoxic effector molecules of population B. Strikingly, samples from individuals that suffer from active Crohn disease displayed dramatically enhanced frequencies of granulysin expressing effector CD127+CD94+ ILCs compared to non-inflamed controls.

2021-10-13 | GSE173642 | GEO

CD127+ CD94+ innate lymphoid cells expressing granulysin and perforin are expanded in patients with Crohn's disease.

Project description:Phenotypic definition of helper ILC1 and NK cells is problematic due to overlapping markers. Recently we showed the identification of cytotoxic ILC3s characterized by expression of CD94. Here we analyse CD127+ ILCs and NK cells in intestinal lamina propria from healthy donors and Crohn's disease patients and identify two populations of CD127+CD94+ ILCs, designated population A and B, that can be distinguished on the expression of CD117, CD18 and cytotoxic molecules. Population B expresses granulysin, a cytotoxic molecule linked to bacterial lysis and/or chemotaxis of monocytes. Granulysin protein is secreted by population B cells upon stimulation with IL-15. Activation of population B in the presence of TGF-β strongly reduces the expression of cytotoxic effector molecules of population B. Strikingly, samples from individuals that suffer from active Crohn's disease display enhanced frequencies of granulysin-expressing effector CD127+CD94+ ILCs in comparison to controls. Thus this study identifies group 1 ILC populations which accumulate in inflamed intestinal tissue of Crohn's disease patients and may play a role in the pathology of the disease.

| S-EPMC8494908 | biostudies-literature

CD94-CD56dim, CD94+CD56dim and CD94+CD56hi natural killer (NK) cell comparison

Project description:CD94-CD56dim, CD94+CD56dim and CD94+CD56hi natural killer (NK) cell comparison

| PRJNA504633 | ENA

CD94-CD56dim, CD94+CD56dim and CD94+CD56hi natural killer (NK) cell chromatin histone modification assay

Project description:CUT and RUN was performed for 3 NK populations and found CD94+CD56hi NK cells exhibit unique H3K4me1, H3K4me4 enriched region, we also identified TCF7 binding region in CD94+CD56hi NK cells.

2019-12-10 | GSE122549 | GEO

Identification of human cytotoxic ILC3s

Project description:Human ILCs are classically categorized into five subsets; cytotoxic CD127-CD94+ NK cells and non-cytotoxic CD127+CD94-, ILC1s, ILC2s, ILC3s and LTi cells. Here, we identify a novel subset within the CD127+ ILC population, characterized by the expression of the cytotoxic marker CD94. These CD94+ ILCs strongly resemble conventional ILC3s in terms of phenotype, transcriptome and cytokine production, but are highly cytotoxic. IL-15 was unable to induce differentiation of CD94+ ILCs towards mature NK cells. Instead, CD94+ ILCs retained RORγt, CD127 and CD200R expression and produced IL-22 in response to IL-15. Culturing non-cytotoxic CD127+ ILC1s or ILC3s with IL-12 induced upregulation of CD94 and cytotoxic activity, effects that were not observed with IL-15 stimulation. Thus, human helper ILCs can acquire a cytotoxic program without differentiating into NK cells.

2020-09-14 | GSE147231 | GEO

CD94-CD56dim, CD94+CD56dim and CD94+CD56hi natural killer (NK) cell comparison

Project description:We compared transcriptomes of 3 NK populations and found CD94+CD56hi NK cells exhibit unique transcriptome.

2019-12-10 | GSE122324 | GEO

CD94-CD56dim, CD94+CD56dim and CD94+CD56hi natural killer (NK) cell chromatin accessibility assay

Project description:CD94-CD56dim, CD94+CD56dim and CD94+CD56hi natural killer (NK) cell chromatin accessibility assay

| PRJNA505524 | ENA

CD94-CD56dim, CD94+CD56dim and CD94+CD56hi natural killer (NK) cell chromatin accessibility assay

Project description:ATAC-Seq was performed for 3 NK populations and found CD94+CD56hi NK cells exhibit unique chromatin accessible region

2019-12-10 | GSE122548 | GEO

CD94-CD56dim, CD94+CD56dim and CD94+CD56hi natural killer (NK) cell chromatin histone modification assay

Project description:CD94-CD56dim, CD94+CD56dim and CD94+CD56hi natural killer (NK) cell chromatin histone modification assay