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Paired Metabolomic and Transcriptomic Time Series of Streptomyces coelicolor M145 Secondary Metabolism

ABSTRACT: Paired Metabolomic and Transcriptomic Time Series of Streptomyces coelicolor M145 Secondary Metabolism

PROVIDER: PRJNA728707 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14483207_1.fastq.gz	Fastqsanger.gz
	SRR14483207_2.fastq.gz	Fastqsanger.gz
	SRR14483210_1.fastq.gz	Fastqsanger.gz
	SRR14483210_2.fastq.gz	Fastqsanger.gz
	SRR14483214_1.fastq.gz	Fastqsanger.gz

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S coelicolor ∆argR vs S. coelicolor M145

Project description:S. coelicolor M145 and S. coelicolor ∆argR were grown in MG medium and samples from 3 biological replicates were taken at 32, 42, 49, 56 and 66 hours.

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Effects of cAMP supplementation on the transcriptome of Streptomyces coelicolor M145 strain

Project description:To identify unique gene expression in cAMP supplemented Streptomyces coelicolor M145 strain. The genes with different gene expression might be key genes to understand the effects of cAMP supplementation on the transcriptome of Streptomyces coelicolor M145.

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Paired Metabolomic and Transcriptomic Time Series of Streptomyces coelicolor M145 Secondary Metabolism

Project description:We utilized weighted correlation networking analysis to integrate metabolite and transcription data. We demonstrate the effectiveness of this strategy and identify potential regulatory effects on biosynthetic gene clusters.

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Evidence of a role for CutRS and actinorhodin in the secretion stress response in Streptomyces coelicolor M145

Project description:This work was carried out to elucidate the proteins that are regulated by the two-component system CutRS in Streptomyces coelicolor M145 and how this response changes in the presence of glucose. A comparison of the whole cell proteomes of Streptomyces coelicolor M145 WT and Streptomyces coelicolor M145 ∆cutRS on both DNA (no glucose) and DNAD (with glucose) was made.

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S. coelicolor M145 SMM medium time course study

Project description:This SuperSeries is composed of the following subset Series: GSE22394: S. coelicolor M145 SMM medium time course study (Culture # 1) GSE22395: S. coelicolor M145 SMM medium time course study (Culture # 2) GSE22397: S. coelicolor M145 SMM medium time course study (Culture # 3) Refer to individual Series

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Streptomyces coelicolor strain:M145

Project description:Cytosine base editing data for Streptomyces coelicolor M145

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Time-series transcription microarray data of Streptomyces coelicolor M145

Project description:The present work aimed at providing noval reference genes (RGs) for streptomycetes by global quantitative analysis of gene expression profile. By using the time-series microarray data obtained in different culture medium (SMM in the present work and modified R5 from publication), the stably expressed genes of S. coelicolor were screened. Further statistical, bioinformatic and biological function analysis picked out 13 candidate RGs. According to qRT-PCR assays, 5 genes with high stability were selected and used for validation in other streptomycetes to assess their prevalence. Additionally, the absolute gene expression level reflcted by RNA-seq was also taken into consideration in the present work to guide the appropriate RGs selection for streptomycetes. Transcriptional profiling of S. coelicolor M145 at different growth phases (exponential, transitional and stationary phase)

2013-12-31 | E-GEOD-53562 | biostudies-arrayexpress

Differential expression of S. coelicolor M145 vs. tdd8 mutants in R5

Project description:Differential expression data set of S. coelicolor M145, tdd8 deletion mutant and overexpressed tdd8 mutant cultured in R5. RNA samples were collected at the visual onset Red antibiotic production following by cDNA synthesis and labeling. The hybridization was conducted using DNA 104K microarrays (Surrey) using gDNA from S. coelicolor M145 as reference.

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Differential expression of S. coelicolor M145 vs. an abrC3 mutant in NB

Project description:Differential expression data set of S. coelicolor M145 and a abrC3 mutant cultured in NB. RNA samples were collected at 36, 48 and 60 hours following cDNA synthesis and labeling. The hybridization was conducted using DNA 104K microarrays (Surrey) using gDNA from S. coelicolor M145 as reference.

2014-01-14 | E-MTAB-1453 | biostudies-arrayexpress

Streptomyces coelicolor cells during various developmental stages: wildtype M145 vs. ÎSCO0204.

Project description:Global transcriptional profiling of the SCO0204 null mutant of Streptomyces coelicolor M145 in comparison to the wildtype M145. Goal was to determine the role of SCO0204.

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