Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide R-loop distribution in Drosophila photoreceptor neurons throughout aging

ABSTRACT: Genome-wide R-loop distribution in Drosophila photoreceptor neurons throughout aging

PROVIDER: PRJNA730035 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR14551654_1.fastq.gz	Fastqsanger.gz
	SRR14551654_2.fastq.gz	Fastqsanger.gz
	SRR14551655_1.fastq.gz	Fastqsanger.gz
	SRR14551655_2.fastq.gz	Fastqsanger.gz
	SRR14551656_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 20

Similar Datasets

Genome-wide R-loop distribution in Drosophila photoreceptor neurons throughout aging

Project description:We report the application of R-loop mapping in a cell-type specific context using photoreceptor neurons at different ages, as measured by days post eclosion (Day 10, Day 30 and Day 50). To validate our approach, we also performed R-loop mapping in 10 day old nuclei that were treated with RNAse H1 for 3 hours prior to application of MapR.

2021-12-31 | GSE174488 | GEO

FANC1 affects R-loop distribution resulting in tumor progression in LUAD

Project description:To plot the R-loop landscape of LUAD cells, we conducted DRIP-seq using S9.6, an anti-DNA/RNA hybrid antibody.We next tested the effect of FANCI deficiency on R-loop distribution and found that FANCI knockout clearly altered the R-loop landscape, showing a strong loss trend.Our analysis revealed that changes in R-loop distribution mediated by FANCI deficiency blocks the activity of the Ras signaling pathway, thereby suppressing tumor-cell proliferation and dissemination. Importantly, our study highlights the causal role of FANCI-mediated changes in R-loop distribution that leads to LUAD development

2023-11-23 | GSE248308 | GEO

Transcriptomic profiling of microglia and astrocytes throughout aging

Project description:Transcriptomic profiling of microglia and astrocytes throughout aging

| PRJNA564299 | ENA

Aging-declined RNA Exportation Impairs Hematopoietic Stem Cells by Inducing R-Loop

Project description:It is well acknowledged that aging-induced DNA damage accumulation impairs hematopoietic stem cell (HSC). While, how DNA damage is accumulated and how to eliminate it to rejuvenate aged HSCs are largely unknown. In this study, we observed that RNA exportation is compromised in aged HSCs due to aging-declined Alyref, the main component of TREX (transcription/export) complex. Targeted dysfunction of Alyref leads to RNA lingering in the nucleus and recapitulates the function of aged HSCs. Mechanistically, RNA accumulation in nucleus results in the formation of RNA:DNA hybrids, called R-loop structures, and subsequent induction of replication stress. Genome-wide profiling reveals significant increase of R-Loop in aged HSCs, which is significantly associated with resident RNA in the nucleus. Quantitative refill of Alyref in aged HSCs significantly restores RNA shuttling, dampens R-Loop and replication stress, and ultimately ameliorates the function of aged HSCs. In brief, our study for the first time demonstrates that aging-induced replication stress originates from the formation of aberrant RNA shuttling-driven R-loop structures and raises the possibility that quantitatively manipulating Alyref signaling can counter the physiologically deleterious effect of aging on HSCs.

2026-01-14 | GSE239299 | GEO