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ABSTRACT: raw sequence of rhizosphere flue-cured tobacco

PROVIDER: PRJNA732105 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14626365.fastq.gz	Fastqsanger.gz
	SRR14626366.fastq.gz	Fastqsanger.gz
	SRR14626367.fastq.gz	Fastqsanger.gz
	SRR14626368.fastq.gz	Fastqsanger.gz
	SRR14626369.fastq.gz	Fastqsanger.gz

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Project description:Flue cured tobacco metagenome

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WGCNA Analysis and Identification of Key Genes in Tobacco in Response to Different Nitrogen Levels

Project description:Background :Nitrogen (N) supply directly impacts growth and quality in flue-cured tobacco. To decipher molecular responses to N gradients, we integrated transcriptomics and weighted gene co-expression network analysis (WGCNA) on leaves from four N treatments: 0 (inherent soil fertility), 60 (low), 105 (standard), and 150 kg/hm² (high). Results :Phenotypic analysis revealed dose-dependent increases in leaf nitrogen content with higher N application, accompanied by excessive vegetative growth and delayed maturity at 150 kg/hm². Transcriptome sequencing identified 47,216 genes, with differentially expressed genes (DEGs) increasing linearly with N levels (1,458–2,147 DEGs). Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment highlighted nitrogen metabolism pathways, yielding 14 DEGs (11 in assimilation, 3 in transport). Weighted gene co-expression network analysis (WGCNA) uncovered two modules (lightcyan1 and black) strongly associated with N responses, harboring transcription factors NtERF11 (AP2/ERF), NtWRKY3 (WRKY), and NtSRM1 (MYB). Sub-network analysis within these modules identified five hub genes: NtGLN1-1, two uncharacterized genes, NtDFC , and NtGDSL. NtGDSL may enhance nitrogen use efficiency (NUE) through stress-responsive mechanisms, while NtDFC could integrate N signaling with developmental processes. These findings provide novel insights into N regulatory networks in flue-cured tobacco. Conclusions :This study reveals the effects of nitrogen application rates on flue-cured tobacco growth and gene expression. By identifying key transcription factors and genes regulating nitrogen metabolism, it provides a theoretical basis for dissecting nitrogen regulatory mechanisms, optimizing fertilization strategies, and improving nitrogen use efficiency in tobacco production.

2025-03-17 | GSE274518 | GEO

Sample of bacteria on the surface of flue-cured tobacco

Project description:Sample of bacteria on the surface of flue-cured tobacco Raw sequence reads

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Metabolomic and Proteomic and Revealed Differences in the Distribution and Synthesis Mechanism of Aroma Precursors in Tobacco Leaf, Stem, and Root at Seedling Stage

Project description:Tobacco, as an important cash crop and model plant, has been studied and explored in various aspects. In China, Yunyan 87 was recognized as a flue-cured tobacco variety and had been widely concerned due to its excellent product quality characteristics. The quality of tobacco products depends on the compound collection of tobacco leaves, including pigments, carbohydrates, amino acids, polyphenols and alkaloids. Present study investigated tobacco seedlings, with the assistant of the untargeted metabonomic technology and the label-free proteomic technology to analyze metabolites and proteins differences in leaf, stem, and root groups respectively. From 298 metabolites and 4993 proteins obtained, there were significant differences in both primary and secondary metabolism involved aroma precursors biosynthesis in seedling tobacco leaves, stems, and roots, such as carbohydrate metabolism, energy metabolism, and amino acid biosynthesis, and secondary metabolism phenylpropanoids, flavonoids and alkaloid biosynthesis in this study. Especially alkaloids metabolites identification results showed nornicotine, anatabine, anatalline, and myosmine, were significantly higher in tobacco roots than in leaves, and stems at seedling stage.

2022-07-12 | PXD032082 | Pride

Carbon-Nitrogen metabolic balance during curing of Flue-cured tobacco

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soil metagenome

Project description:the effect of different relay intercropping model on bacterial diversity of rhizosphere soil in flue-cured tobacco Raw sequence reads

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Project description:Reducing the nitrate content of burley tobacco by grafting with flue-cured tobacco

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Proteomics analysis of nitrogen stress on the influence of carbon and nitrogen metabolism of flue-cured tobacco

Project description:Nitrogen stress is the main abiotic stress factor affecting the carbon and nitrogen balance of flue-cured tobacco. Tandem mass tag (TMT) proteomics was used to compare the differently expressed proteins (DEPs) of flue-cured tobacco under high- (HN) and low- (LN) nitrogen stress, and Gene Ontology (GO) function annotation, Kyoto Encyclopedia for Genes and Genomes (KEGG) analysis, and protein-protein interaction (PPI) analysis were conducted. The results showed that the difference between LN and HN stress was mainly reflected in the formation pathways of carbon and nitrogen metabolism.

2024-08-22 | PXD055100 |

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