Identification of indoleamine 2, 3-dioxygenase 1 (IDO1) regulated genes in human glioblastoma cell line U87
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ABSTRACT:
PROVIDER: PRJNA733217 | ENA |
REPOSITORIES: ENA
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Project description:Transcriptome analysis of U87 cells under different treatments to identify IDO1-regulated genes Indoleamine 2, 3-dioxygenase 1 (IDO1) is a tryptophan (Trp) catabolic enzyme that converts Trp into downstream kynurinine (Kyn). Many studies have indicated that IDO1 is a critical suppressive immune checkpoint molecule invovled in various types of cancer. Canonically, the underlying mechanism of IDO1 immunosuppressive role is related with its enzyme activity, that is the depletion of Trp and accumulation of Kyn lead to increased tumor infiltrating suppressive regulatory T cells. Recent studies, however, challenged this hypothesis and imply that tumor cell-derived IDO1 can mediate immunosuppression independent of its enzyme activity. In this study, we aim to identify genes that are regulated by IDO1 in human glioblastoma cells, a gene expression regulatory function of IDO1 that is indepent of its enzyme activity.
2021-12-10 | GSE175700 | GEO
Project description:Aryl hydrocarbon receptor (AHR) activation by tryptophan (Trp) catabolites enhances tumor malignancy and suppresses anti-tumor immunity. Hitherto, indoleamine-2,3-dioxygenase 1 (IDO1) or tryptophan- 2, 3-dioxygenase (TDO2) are recognized as the main Trp-catabolizing enzymes (TCEs) responsible for the generation of AHR agonists. Here, the ability of the aromatic L-amino acid oxidase, interleukin 4 induced 1 (IL4I1), to activate the AHR was investigated using IL4I1 knockout CAS-1 glioblastoma cells.
2020-08-19 | GSE149910 | GEO
Project description:Early acute rejection of human allografts is mediated by circulating alloreactive host effector memory T cells (TEM). TEM infiltration typically occurs across graft post-capillary venules and involves sequential interactions with graft-derived endothelial cells (ECs) and pericytes (PCs). While the role of ECs in allograft rejection has been extensively studied, contributions of PCs to this process are largely unknown. This study aimed to characterize the effects and mechanisms of interactions between human PCs and allogeneic TEM. We report that unstimulated PCs, like ECs, can directly present alloantigen to TEM but while IFN-γ-activated ECs (γ-ECs) show increased ability to stimulate alloreactive T cells, IFN-γ-activated PCs (γ-PCs) instead suppress TEM proliferation but not cytokine production or signaling. RNA sequencing analysis of PCs, γ-PCs, ECs, and γ-ECs reveal induction of indoleamine 2,3-dioxygenase 1 (IDO1) in γ-PCs to significantly higher levels than in γ-ECs that correlates with tryptophan depletion in vitro. Consistently, shRNA knockdown of IDO1 markedly reduces -PC-mediated immunoregulatory effects. Adoptively transferred T cells induced PC expression of IDO1 in allogeneic human skin grafts on immunodeficient mice. We conclude that immunosuppressive properties of human PCs are not intrinsic but instead result from IFN-γ-induced IDO1-mediated tryptophan depletion.
2018-05-09 | GSE109322 | GEO
Project description:Rattus norvegicus Ido1, indoleamine 2,3-dioxygenase 1 [Source:RGD Symbol;Acc:619989], is differentially expressed in 12 experiment(s);
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Project description:Rattus norvegicus Ido1, indoleamine 2,3-dioxygenase 1 [Source:RGD Symbol;Acc:619989], is expressed in 2 baseline experiment(s);
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Project description:Sus scrofa IDO1, indoleamine 2,3-dioxygenase 1 [Source:VGNC Symbol;Acc:VGNC:96371], is differentially expressed in 5 experiment(s);
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Project description:Macaca mulatta IDO1, indoleamine 2,3-dioxygenase 1 [Source:VGNC Symbol;Acc:VGNC:73580], is differentially expressed in 1 experiment(s);
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Project description:Macaca mulatta IDO1, indoleamine 2,3-dioxygenase 1 [Source:VGNC Symbol;Acc:VGNC:73580], is expressed in 3 baseline experiment(s);
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Project description:Homo sapiens IDO1, indoleamine 2,3-dioxygenase 1 [Source:HGNC Symbol;Acc:HGNC:6059], is expressed in 32 baseline experiment(s);
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Project description:Sus scrofa IDO1, indoleamine 2,3-dioxygenase 1 [Source:VGNC Symbol;Acc:VGNC:96371], is expressed in 1 baseline experiment(s);
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