Project description:Small RNAs (21-24 nt) are pivotal regulators of gene expression that guide both transcriptional and post-transcriptional silencing mechanisms in diverse eukaryotes, including most if not all plants. MicroRNAs (miRNAs) and short interfering RNAs (siRNAs) are the two major types, both of which have a demonstrated and important role in plant development, stress responses and pathogen resistance. In this work, we used a deep sequencing approach (Sequencing-By-Synthesis, or SBS) to develop sequence resources of small RNAs from different Cycas rumphii tissues (leaves, ovules, and microsporangia). The high depth of the resulting datasets enabled us to examine in detail critical small RNA features as size distribution, tissue-specific regulation and sequence conservation between different organs in this species. We also developed database resources and a dedicated website (http://smallrna.udel.edu/) with computational tools for allowing other users to identify new miRNAs or siRNAs involved in specific regulatory pathways, verify the degree of conservation of these sequences in other plant species and map small RNAs on genes or larger regions of the maize genome under study.

2011-05-10 | GSE28961 | GEO

Genetic characterization of the entire range of Cycas panzhihuaensis (Cycadaceae).

Project description:Cycas panzhihuaensis L. Zhou & S. Y. Yang (Cycadaceae) is an endangered gymnosperm species endemic to the dry-hot valley of the Jinsha River basin in southwest China. Although the wild C. panzhihuaensis population from Panzhihua Cycad Natural Reserve is well protected and its genetic diversity has been well assessed, the genetic characteristics of populations outside the nature reserve, which face larger risks of extinction, remain unknown. Furthermore, the population genetics and historical dynamics of this endemic and endangered species have not been examined across its entire range. In this study, to analyze the genetic diversity, phylogeographical structure and demographic history of C. panzhihuaensis from all its seven known locations, we sequenced and compared molecular data from chloroplastic DNA (psbA-trnH, psbM-trnD, and trnS-trnG), single-copy nuclear genes (PHYP, AC5, HSP70, and AAT) from 61 individuals, as well as 11 nuclear microsatellite loci (SSR) from 102 individuals. We found relatively high genetic diversity within populations and high genetic differentiation among populations of C. panzhihuaensis, which is consistent with the patterns of other Asian inland cycads. Although no significant phylogeographical structure was detected, we found that small and unprotected populations possess higher genetic diversity and more unique haplotypes, which revises our understanding of diversity within this species and deserves due attention. Analysis of demographic dynamics suggest that human activity might be the key threat to C. panzhihuaensis. Based on the genetic characterization of C. panzhihuaensis, we propose several practical guidelines for the conservation of this species, especially for the populations with small sizes.

| S-EPMC7046506 | biostudies-literature

Cycas sphaerica isolate:Leaf

Project description:Cycas sphaerica Roxb.genetic diversity

| PRJNA493555 | ENA