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Early life stress affects the miRNA cargo in epididymal extracellular vesicles in mouse

ABSTRACT: Early life stress affects the miRNA cargo in epididymal extracellular vesicles in mouse

PROVIDER: PRJNA734436 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14714433.fastq.gz	Fastqsanger.gz
	SRR14714434.fastq.gz	Fastqsanger.gz
	SRR14714435.fastq.gz	Fastqsanger.gz
	SRR14714436.fastq.gz	Fastqsanger.gz
	SRR14714437.fastq.gz	Fastqsanger.gz

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Early life stress affects the miRNA cargo in epididymal extracellular vesicles in mouse

Project description:Sperm RNA can be modified by environmental factors and has been implicated in communicating signals about changes in a father's environment to the offspring. The RNA composition of sperm is influenced during its final stage of maturation in the epididymis by extracellular vesicles released by epididymal cells. We studied the effect of exposure to stress in postnatal life on the transcriptome of epididymal extracellular vesicles using a mouse model of transgenerational transmission. We found that the small RNA signature of epididymal extracellular vesicles, particularly miRNAs, is altered in adult males exposed to postnatal stress. miRNAs changes correlate with differences in the expression of their target genes in sperm and zygotes generated from that sperm. These results suggest that stressful experiences in early life can have persistent biological effects on the male reproductive tract that may in part be responsible for the transmission of the effects of exposure to the offspring.

2021-07-31 | GSE175976 | GEO

miRNA Cargo of Matrix-Bound Extracellular Vesicles Isolated from Human Left Ventricular Tissue Differs with Heart Failure

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Profiling RNA Cargo in Extracellular Vesicles from hiPSC-Derived Neurons of Alzheimer’s Disease Patients

Project description:Alzheimer’s disease (AD) is a major neurodegenerative disorder that affects more than 55 million people, with an incidence that is projected to triple by 2050. Despite continuous advancements in the field, reliable treatment and early detection strategies remain elusive. Extracellular vesicles (EVs) play a major role in cellular communication throughout the body. In this study, we assessed the cargo of neuronal-specific EVs for their potential as AD biomarkers. We isolated EVs released from iPSC-derived excitatory glutamatergic neurons generated from eight AD patients (ADiNEVs) and six healthy controls (iNEVs). We performed RNA-sequencing and identified significant differences in RNA cargo between ADiNEVs and iNEVs. Notably, fewer small nuclear RNAs (snRNAs) were found in ADiNEVs. RNA transcripts significantly more abundant in ADiNEVs included MT-CO1, PRR32, and IGSF8 messenger RNAs. We also observed fewer XIST long noncoding RNAs and miR-7-5p microRNA content in ADiNEVs. These findings suggest that precision medicine approaches, such as characterizing the content of EVs from a patient’s own cells, could advance early detection and management of AD.

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Isolation and next generation sequencing of epididymal extracellular vesicles reveals segmental patterns of miRNA expression and a potential mechanism for modification of the sperm epigenome.

Project description:Interest in the sperm epigenome continues to grow in view of the realization that it is vulnerable to dynamic modifications arising from a variety of paternal environment exposures and that this legacy can serve as an important determinant of intergenerational inheritance. It has been postulated that such exchange may be communicated to maturing populations of spermatozoa via the transfer of small non-coding RNAs in a mechanism involving epididymosomes; small membrane bound vesicles secreted by the soma of the male reproductive tract (epididymis). Herein we confirm that mouse epididymosomes encapsulate an impressive repertoire of >350 microRNA (miRNA), a majority (~60%) of which are also represented in the miRNA signature of maturing spermatozoa. This includes >50 miRNAs that were found exclusively in epididymal sperm and epididymosomes, but not in the surrounding soma, thus supporting the notion that epididymosomes may selectively deliver regulatory non-coding RNA cargo to maturing sperm. Interestingly, we also documented substantial plasticity in terms of the epididymosome-borne miRNAs, including significant fold changes in the relative abundance of almost half of the miRNAs along the length of the epididymis. Finally, we provide the first direct evidence for the transfer of several prominent miRNAs between mouse epididymosomes and spermatozoa, and in so doing afford novel insight into a mechanism of intercellular communication through which the RNA payload of sperm can be modified during their post-testicular development. Examination of the microRNA expression profile of mouse epididymal epididymosomes using next generation sequencing in triplicate.

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Blood flow-restricted resistance exercise (BFRE) alters the surface profile, miRNA cargo and functional impact of circulating extracellular vesicles

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Circadian rhythm does not affect the miRNA cargo of bovine raw milk extracellular vesicles

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