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The Hippo pathway regulates density-dependent proliferation of iPSC-derived cardiac myocytes

ABSTRACT: The Hippo pathway regulates density-dependent proliferation of iPSC-derived cardiac myocytes

PROVIDER: PRJNA735115 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14730171_1.fastq.gz	Fastqsanger.gz
	SRR14730171_2.fastq.gz	Fastqsanger.gz
	SRR14730172_1.fastq.gz	Fastqsanger.gz
	SRR14730172_2.fastq.gz	Fastqsanger.gz
	SRR14730173_1.fastq.gz	Fastqsanger.gz

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The Hippo pathway regulates density-dependent proliferation of iPSC-derived cardiac myocytes

Project description:Inducing cardiac myocytes to proliferate is considered a potential therapy to target heart disease, however, modulating cardiac myocyte proliferation has proven to be a technical challenge. The Hippo pathway is a kinase signaling cascade that regulates cell proliferation during the growth of the heart. Inhibition of the Hippo pathway increases the activation of the transcription factors YAP/TAZ, which translocate to the nucleus and upregulate transcription of pro-proliferative genes. The Hippo pathway regulates the proliferation of cancer cells, pluripotent stem cells, and epithelial cells through a cell-cell contact-dependent manner, however, it is unclear if cell density-dependent cell proliferation is a consistent feature in cardiac myocytes. Here, we used cultured human iPSC-derived cardiac myocytes (hiCMs) as a model system to investigate this concept. hiCMs have a comparable transcriptome to the immature cardiac myocytes that proliferate during heart development in vivo. Our data indicate that a dense syncytium of hiCMs can regain cell cycle activity and YAP expression and activity when plated sparsely or when density is reduced through wounding. We found that combining two small molecules, XMU-MP-1 and S1P, increased YAP activity and further enhanced proliferation of low-density hiCMs. Importantly, these compounds had no effect on hiCMs within a dense syncytium. These data add to a growing body of literature that link Hippo pathway regulation with cardiac myocyte proliferation and demonstrate that regulation is restricted to cells with reduced contact inhibition.

2021-09-08 | GSE176142 | GEO

The Hippo pathway regulates density-dependent proliferation of iPSC-derived cardiac myocytes.

| S-EPMC8423799 | biostudies-literature

Gene expression after knockdown of transcription factors in human iPSC-derived cardiac myocytes

Project description:We report gene expression changes after knockdown of transcription factors in human iPSC-derived cardiac myocytes. In prior experiments we showed that transcription factors known to be important for cardiac development were frequently up-regulated (i.e., "responsive") after small molecule perturbations of cultured iPSC-derived cardiac myocytes. We used RNA sequencing to test whether siRNA-mediated knockdown of transcription factors with different perturbation-responsiveness and tissue-specificity profiles would lead to inappropriate up-regulation of non-myocyte gene sets in cardiac myocytes.

2021-02-17 | GSE166823 | GEO

Perturbation panel profiling of human iPSC-derived cardiac myocytes and primary dermal fibroblasts

Project description:We report gene expression by RNAtag-seq after treatment with 75 different small molecule perturbations in culture of human iPSC-derived cardiac myocytes and genetically matched primary dermal fibroblasts. Perturbations were chosen from the SelleckChem Bioactive Library, among all molecules targeting any kinases or G-protein coupled receptors, and chosen to have as little overlap in annotated targets as possible. Based on these experiments (and others) we show that transcription factors important for cardiac development and cardiac myocyte identity maintenance were frequently up-regulated (i.e., "responsive") after small molecule perturbations of cultured iPSC-derived cardiac myocytes (i.e., responsive). We also show that the set of highly responsive transcription factors in fibroblasts are enriched for barriers to fibroblast reprogramming to iPSC.

2021-02-20 | GSE167128 | GEO

Mitochondria regulate proliferation in adult cardiac myocytes

Project description:Mitochondria regulate proliferation in adult cardiac myocytes

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The regulatory role of miR-365 in human iPSC-derived cardiac myocytes

Project description:The regulatory role of miR-365 in human iPSC-derived cardiac myocytes

| PRJNA770437 | ENA

Gene expression after knockdown of transcription factors in human iPSC-derived cardiac myocytes

Project description:Gene expression after knockdown of transcription factors in human iPSC-derived cardiac myocytes

| PRJNA702074 | ENA

Perturbation panel profiling of human iPSC-derived cardiac myocytes and primary dermal fibroblasts

Project description:Perturbation panel profiling of human iPSC-derived cardiac myocytes and primary dermal fibroblasts

| PRJNA703032 | ENA

Transcription profiling of H2O2 treated rat cardiac myocytes

Project description:Title: Changes in gene expression affected by H2O2 in cardiac myocytes. Description: We aim to identify the changes in gene expression in response to oxidative stress in rat neonatal ventricular myocytes. Oxidative stress will be induced by dosing neonatal ventricular myocyte cultures with 0.2, 0.1 and 0.04mM hydrogen peroxide at 2, 4 and 8 hr time points using unstimulated myocytes as control.

2011-11-08 | E-MIMR-12 | biostudies-arrayexpress

Wnt5a-YAP signaling axis mediates mechanotransduction in cardiac myocytes and contributes to the transition to heart failure (rat sample)

Project description:Non-canonical Wnt signaling activated by Wnt5a and Wnt11 is required for the development of second heart field cardiac progenitor cells in mice. However, the pathophysiological role of non-canonical Wnt signaling in the adult heart has not been fully elucidated. Here we show that cardiomyocyte-specific Wnt5a knockout mice exhibit improved systolic function and reduced expression of mechanosensitive genes including Nppb compared to control mice when subjected to pressure overload. In cultured cardiac myocytes, Wnt5a knockdown reduces the upregulation of Nppb gene expression and YAP nuclear translocation induced by cyclic cell stretch. Wnt5a knockdown-induced Nppb downregulation in response to cell stretch is rescued by inhibition of Hippo pathway, and the rescue effect of Hippo inhibition is canceled by YAP knockdown. These results collectively suggest that Wnt5a-YAP signaling axis mediates mechanotransduction in cardiac myocytes and contributes to the transition to heart failure.

2023-07-17 | GSE200666 | GEO

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