Project description:Malignant neoplasms arise within a region of chronic inflammation caused by tissue injuries. Inflammation is a key factor involved in all aspects of tumorigenesis including initiation, proliferation, invasion, angiogenesis, and metastasis. Interleukin-1 (IL-1) plays critical functions in tumor development with influencing the tumor microenvironment and promoting cancer progression. RNA sequencing analysis performed with HaCaT-HRasG12V cells, HaCaT-HRasG12V-THG-1 (WT) cells and HaCaT-HRasG12V THG-1 (S264A mutant) cells revealed that THG-1 overexpression enhances the transcription of NF-κB targets including IL1A, IL1B, TNFA, and IL8.
Project description:We addressed the optimization of the xylanolytic activity in extracellular enzymatic extracts of Cellulomonas sp. B6 and C. fimi B-402 for their subsequent application in lignocellulosic biomass hydrolysis, by culture in several substrates. As demonstrated by secretomic profiling, wheat bran and waste paper resulted to be suitable inducers for the secretion of xylanases of Cellulomonas sp. B6 and C. fimi B-402, respectively. Both strains showed high xylanolytic activity in culture supernatant although Cellulomonas sp. B6 was the most efficient xylanolytic strain.