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CUT-tag analysis of C1qbp sufficient and deficient CD8+ T cells

ABSTRACT: CUT-tag analysis of C1qbp sufficient and deficient CD8+ T cells

PROVIDER: PRJNA736022 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR14760076_1.fastq.gz	Fastqsanger.gz
	SRR14760076_2.fastq.gz	Fastqsanger.gz
	SRR14760077_1.fastq.gz	Fastqsanger.gz
	SRR14760077_2.fastq.gz	Fastqsanger.gz
	SRR14760078_1.fastq.gz	Fastqsanger.gz

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CUT-tag analysis of C1qbp sufficient and deficient CD8+ T cells

Project description:This study is aimed to Investigate whether C1qbp deficiency affects the histone modification during the differentiation stage of CD8+ T cells. Transferred WT and C1qbp KO P14 cells sorted from the spleen of donor mice on day 5 post-infection with LCMV Armstrong were collected and treated for CUT-tag. CUT-tag-Seq analysis provided evidence of histone modification changes between WT and C1qbp KO CD8+ T cells. Our study shows that on day 5 post-infection with LCMV Armstrong, along with mRNA expressing profiling, the CUT-tag-seq exhibits obvious changes in H3K27me3 and H3K27Ac modification of indicated genes of WT and C1qbp KO CD8+ T cells.

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ATAC-seq analysis of C1qbp sufficient and deficient CD8+ T cells sorted from mice post infection with LCMV Armstrong

Project description:This study is aimed to explore whether C1qbp deficiency affects the epigenetic modification during the differentiation stage of CD8+ T cells. Transferred WT and C1qbp KO P14 cells sorted from the spleen of donor mice on day 5 post-infection with LCMV Armstrong were collected and treated for ATAC-seq. ATAC-Seq analysis provided sufficient but not necessary evidence of epigenetic changes between WT and C1qbp KO CD8+ T cells. Our study have shown that, on day 5 post-infection with LCMV Armstrong, even WT and C1qbp KO CD8+ T cells have different mRNA expressing profiling, the ATAC-seq shows slight changes in chromatin accessibility of indicated genes.

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