Project description:Reinke's edema (RE) is a laryngeal lesion related to excessive tobacco smoking, voice overuse, and laryngopharyngeal reflux. Although the risk of malignancy is low, RE has been classified among premalignant lesions. Array-based comparative genomic hybridization (aCGH) was used to investigate DNA copy number alterations (CNA) and as a tool to identify markers of risk stratification.
Project description:Epigenetics may play an important role in the occurrence and development of high-altitude pulmonary edema. To investigate the DNA methylation driving genes associated with high-altitude pulmonary edema, we also performed reduced representation bisulfite sequencing (RRBS) for blood identify differences of DNA methylation status of 10 HAPE patients and 10 healthy volunteers.
Project description:Edema toxin (EdTx), which is a combination of edema factor and a binding moiety (protective antigen), is produced by Bacillus anthracis, the etiological agent of anthrax. EdTx is an adenylyl cyclase enzyme that converts adenosine triphosphate to adenosine-3’,5’-monophosphate, resulting in interstitial edema seen in anthrax patients. We used GeneChip analysis to examine global transcriptional profiles of EdTx-treated RAW 264.7 murine macrophage-like cells at 3 and 6 hr. Keywords: Toxin response
Project description:High Altitude Pulmonary Edema (HAPE) is a disease that interacts with environmental and epigenetic factors with a severe clinical course and high mortality rate. This study aims to investigate DNA methylation alterations of HAPE and identify the driver genes. The Illumina Infinium MethylationEPIC BeadChip (850K) was used to obtain DNA methylation profiles across 860,000 CpGs in blood of 6 HApe patients and 6 healthy volunteers.