Project description:Reinke's edema (RE) is a laryngeal lesion related to excessive tobacco smoking, voice overuse, and laryngopharyngeal reflux. Although the risk of malignancy is low, RE has been classified among premalignant lesions. Array-based comparative genomic hybridization (aCGH) was used to investigate DNA copy number alterations (CNA) and as a tool to identify markers of risk stratification.
Project description:Epigenetics may play an important role in the occurrence and development of high-altitude pulmonary edema. To investigate the DNA methylation driving genes associated with high-altitude pulmonary edema, we also performed reduced representation bisulfite sequencing (RRBS) for blood identify differences of DNA methylation status of 10 HAPE patients and 10 healthy volunteers.
Project description:Cerebral edema, a life-threatening consequence of central nervous system (CNS) injury, lacks effective therapies. Aquaporin-4 (AQP4), the main water channel in astrocytes, plays critical roles in both the formation and resolution of edema, with unclear regulatory mechanisms. Here, we uncover a γ-secretase-independent function of presenilin enhancer 2 (Pen-2) in AQP4 turnover. Conditional Pen-2 inactivation in astrocytes results in metabolic alterations, severe cerebral edema and early lethality. Mechanistically, Pen-2 physically interacts with AQP4 to promote its degradation through the autophagy-lysosome pathway. Treatment with acetazolamide, an AQP4 inhibitor, reverses the edema phenotype in Pen-2-deficient mice, establishing AQP4 as a critical downstream effector. This study unveils a previously unrecognized Pen-2-AQP4 axis that governs brain water homeostasis and reveals a promising therapeutic target for edema treatment.
Project description:Edema toxin (EdTx), which is a combination of edema factor and a binding moiety (protective antigen), is produced by Bacillus anthracis, the etiological agent of anthrax. EdTx is an adenylyl cyclase enzyme that converts adenosine triphosphate to adenosine-3’,5’-monophosphate, resulting in interstitial edema seen in anthrax patients. We used GeneChip analysis to examine global transcriptional profiles of EdTx-treated RAW 264.7 murine macrophage-like cells at 3 and 6 hr. Keywords: Toxin response
Project description:Tear proteome changes in diabetic macular edema with treatment. The constitution of tear protein change was assessed for difference in treatment response