Project description:Recent studies indicated that the NF-kB RelB subunit may play roles beyond immune modulations. The molecular mechanism that may allow RelB to engage in such diverse physiological activities remains unclear. To better understand the RelB-driven transcriptome, therefore, we subjected wild type and Relb-/- mouse embryonic fibroblast (MEF) to microarray analyses using Illumina MouseRef-8 v2.0 Expression BeadChip. We propose that our transcroptomic analyses will help linking RelB to other important cellular pathways.
Project description:We have used primary MEFs derived from wild type and E2F4 null mice growing asynchrounously in serum to generate a signature for E2F4 pathway activation. 10 wild type and 10 E2F4 null samples were each assayed using the Affymetrics Mouse Genome 430A 2.0 array. Keywords: Primary MEFs from wild type and E2F4 null mice
Project description:TICAM1 knockout and wild-type (TICAM1 knockout MEFs with restored TICAM1 expression) MEFs were treated by c-di-GMP or DMSO for 4 hours. Total RNA was analyzed via Illumina Mouse Ref-8 V2. Changes in gene induction, especially of interferon-stimulated genes, between c-di-GMP and DMSO treated cells were examined.
Project description:To explore the effect of Ptenα on antiviral immunity pathway, we conducted RNA transcriptome profiling of wild-type and Ptenα-/- MEFs (mouse embryonic fibroblast cells) in response to VSV (vesicular stomatitis virus)