Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RECEPTOR LIKE PROTEIN KINASE 2 constrains proximal secondary vein branching in the Arabidopsis thaliana embryo

ABSTRACT: RECEPTOR LIKE PROTEIN KINASE 2 constrains proximal secondary vein branching in the Arabidopsis thaliana embryo

PROVIDER: PRJNA737797 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR14817178.fastq.gz	Fastqsanger.gz
	SRR14817179.fastq.gz	Fastqsanger.gz
	SRR14817180.fastq.gz	Fastqsanger.gz
	SRR14817181.fastq.gz	Fastqsanger.gz
	SRR14817182.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 10

Similar Datasets

RECEPTOR LIKE PROTEIN KINASE 2 constrains proximal secondary vein branching in the Arabidopsis thaliana embryo

Project description:Over time, plants have evolved flexible self-organizing patterning mechanisms to adapt tissue functionality to continuous organ growth. A clear example of this process is the multicellular organization of vascular cells into narrow and elongated conductive channels in foliar organs of Arabidopsis thaliana such as cotyledons. The establishment of a closed vascular network is achieved through the coordinated specification of newly recruited procambial cells by means of their proliferation and elongation. An important and yet poorly understood component of this process is secondary vein branching; a mechanism employed to extend vascular tissues throughout cotyledon surface. Here we revise the directionality of the formation of vascular tissues in the embryonic cotyledon of Arabidopsis and show that distal veins arise from the bifurcation of cell files contained in the midvein. Instead, proximal veins emerge from the division of provascular cells, a process partially constrained by RECEPTOR LIKE PROTEIN KINASE 2 (RPK2). Utilizing genetic, transcriptomic and live-cell imaging analyses, we show that RPK2 function is antagonized by COTYLEDON VASCULAR PATTERN 2 and its homologous CVP2 LIKE 1. Whilst RPK2 expression at the cotyledon margin prevents the branching of secondary proximal veins, the divergence of the midvein into distal veins appears to be auxin-dependent and follows a distinct regulatory mechanism. Our work supports a model in which RPK2 modulates vascular complexity independently of cell-to-cell auxin-propagation to adapt the spatial configuration of vascular tissues to organ growth.

2022-06-09 | GSE178241 | GEO

A genetic framework for proximal secondary vein branching in the Arabidopsis thaliana embryo.

Project description:Over time, plants have evolved flexible self-organizing patterning mechanisms to adapt tissue functionality for continuous organ growth. An example of this process is the multicellular organization of cells into a vascular network in foliar organs. An important, yet poorly understood component of this process is secondary vein branching, a mechanism employed to extend vascular tissues throughout the cotyledon surface. Here, we uncover two distinct branching mechanisms during embryogenesis by analyzing the discontinuous vein network of the double mutant cotyledon vascular pattern 2 (cvp2) cvp2-like 1 (cvl1). Similar to wild-type embryos, distal veins in cvp2 cvl1 embryos arise from the bifurcation of cell files contained in the midvein, whereas proximal branching is absent in this mutant. Restoration of this process can be achieved by increasing OCTOPUS dosage as well as by silencing RECEPTOR-LIKE PROTEIN KINASE 2 (RPK2) expression. Although RPK2-dependent rescue of cvp2 cvl1 is auxin- and CLE peptide-independent, distal branching involves polar auxin transport and follows a distinct regulatory mechanism. Our work defines a genetic network that confers plasticity to Arabidopsis embryos to spatially adapt vascular tissues to organ growth.

| S-EPMC9270971 | biostudies-literature

Transcription profiling of branching and non-branching regions of the mouse embryonic lung.

Project description:During development, the proximal and distal regions of respiratory tract undergo distinct processes that ultimately give rise to conducting airways and alveoli. To gain insights into the genetic pathways differentially activated in these regions when branching morphogenesis is initiating, we characterized their transcriptional profiles in murine rudiments isolated at embryonic day 11.5.

2007-09-29 | E-GEOD-1423 | biostudies-arrayexpress

Global analysis of genes differentially expressed in branching and non-branching regions of the mouse embryonic lung

2004-05-23 | GSE1423 | GEO

Proteome of human branching and non-branching cholangiocyte organoids

Project description:Current model systems for studying intrahepatic biliary disease fail to recapitulate the architecture of intrahepatic bile ducts. Organoids from intrahepatic cholangiocytes can with a new culture method grow with a branching pattern resembling the structure of intrahepatic bile ducts. This dataset contains data from samples of these organoids and from control organoids cultured without this technique. Culture have been performed using isotope labeled amino acids to help distinguish cell derived proteins from the basement membrane extract used as a culture substrate for the organoids.

2022-05-09 | PXD028532 | Pride

DNMT1 is required for proximal-distal patterning of the lung endoderm and restraining alveolar type 2 cell fate

Project description:Lung endoderm development occurs through a series of finely coordinated transcriptional processes that are regulated by epigenetic mechanisms. However, the role of DNA methylation in regulating lung endoderm development remains poorly understood. We demonstrate that DNA methyltransferase 1 (Dnmt1) is required for early branching morphogenesis of the lungs and for restraining epithelial fate specification. Loss of Dnmt1 leads to an early branching defect, a loss of proximal endodermal cell differentiation, and an expansion of the distal endoderm compartment. Dnmt1 deficiency also leads to precocious distal endodermal cell differentiation with premature expression of alveolar type 2 cell restricted genes. These data reveal an important requirement for Dnmt1 mediated DNA methylation in early lung development to promote proper branching morphogenesis, maintain proximal endodermal cell fate, and suppress premature activation of the distal epithelial fate.

2020-01-24 | GSE129177 | GEO

Transcriptomic analysis of pulmonary vein under pathological pulmonary vein stenosis

Project description:We reported the RNAseq analyses of pulmonary vein tissues at the age of postnatal day 21 (P21) on SD rats with or without pulmonary vein stenosis. Pulmonary vein stenosis was induced by performing pulmonary vein banding surgery within 24 hours postnatally. RNAseq analyses of the pulmonary vein tissues was generated at P21 from 3 sham-operated rats and 3 pulmonary vein banding rats. The results revealed that there were 1124 differentially expressed genes between PAB and sham group at P7, among which 714 were upregulated and 410 were downregulated.

2024-07-10 | GSE267175 | GEO

Erysiphe necator strain:branching

Project description:Erysiphe necator Genome sequencing branching isolate

| PRJNA248900 | ENA

Transcriptomic analysis of pulmonary vein under pathological pulmonary vein stenosis

Project description:Transcriptomic analysis of pulmonary vein under pathological pulmonary vein stenosis

| PRJNA1110169 | ENA

Single-cell RNA sequencing on the vein wall of mouse deep vein thrombosis

Project description:Deep vein thrombosis (DVT) is a common clinical problem, but its cellular and molecular mechanisms remain incompletely understood. We performed single-cell RNA sequencing (scRNA-seq) on the vein wall of mouse inferior vena cava (IVC) ligation model of deep vein thrombosis (DVT), to analyze the transcriptomic changes in the vein wall during acute venous thrombosis.

2023-02-10 | GSE221978 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data