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Endothelial cells synergize with fibroblasts to form a niche for engineering alveolar epithelium

ABSTRACT: Endothelial cells synergize with fibroblasts to form a niche for engineering alveolar epithelium

PROVIDER: PRJNA738933 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14855048.fastq.gz	Fastqsanger.gz
	SRR14855049.fastq.gz	Fastqsanger.gz
	SRR14855050.fastq.gz	Fastqsanger.gz
	SRR14855051.fastq.gz	Fastqsanger.gz
	SRR14855052.fastq.gz	Fastqsanger.gz

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Endothelial cells synergize with fibroblasts to form a niche for engineering alveolar epithelium

Project description:We perform single-cell RNA sequencing of whole engineered lungs generated either by alveolar epithelial type 2 cell (AEC2)/fibroblast (FB) co-culture or by AEC2/FB/endothelial cell (EC) tri-culture, to investigate AEC2 and FB phenotype in the presence and absence of endothelial cells. Primary postnatal day 7 (P7) AEC2s, P7 lung FBs, and primary rat lung microvascular ECs (from 4-6 week-old rats) used for engineered cultures, as well as native P7 whole rat lung, were sequenced for comparison. We find that native-like AEC2 phenotype, alveolar structure, and cellular signaling patterns are better recapitulated via epithelial-mesenchymal-endothelial tri-culture rather than by a more simplified system. FBs cultured in the presence of endothelial cells demonstrate reduced expression of contractile markers and upregulate features of an AEC2 niche-supportive signature.

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