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The angiopoietin-Tie2 pathway regulates Purkinje cell dendritic morphogenesis in a cell-autonomous manner

ABSTRACT: The angiopoietin-Tie2 pathway regulates Purkinje cell dendritic morphogenesis in a cell-autonomous manner

PROVIDER: PRJNA739970 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14879049.fastq.gz	Fastqsanger.gz
	SRR14879050.fastq.gz	Fastqsanger.gz
	SRR14879051.fastq.gz	Fastqsanger.gz
	SRR14879052.fastq.gz	Fastqsanger.gz
	SRR14879053.fastq.gz	Fastqsanger.gz

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The angiopoietin-Tie2 pathway regulates Purkinje cell dendritic morphogenesis in a cell-autonomous manner

Project description:Neuro-vascular communication is essential to synchronize central nervous system development. Yet, the specific cellular players, defined developmental processes and molecular mechanisms involved in this crosstalk remain poorly characterized. Here we identify the angiopoietin/Tie2 signaling axis as a crucial regulator of dendritic morphogenesis of Purkinje cells (PC) during cerebellum development. We show that in the developing cerebellum Tie2 expression is not restricted to blood vessels but it is also in PCs. Its ligands angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2) are expressed in neural cells and endothelial cells (ECs), respectively. PC-specific deletion of Tie2 results in reduced dendritic arborization, which is recapitulated in neural-specific Ang1-knockout and Ang2 full-knockout mice. Mechanistically, RNA sequencing reveals that Tie2 deficient PCs present alterations in gene expression of multiple genes involved in cytoskeleton organization, dendritic formation, growth and branching. Functionally, those morphological changes are accompanied by alterations in PC network electrophysiology. Altogether, our data propose that the Ang/Tie2 signaling axis serve as mediator of intercellular communication between neural cells, ECs and PCs, required to regulate PC dendritic morphogenesis and function.

2021-12-31 | GSE178659 | GEO

The angiopoietin-Tie2 pathway regulates Purkinje cell dendritic morphogenesis in a cell-autonomous manner.

Project description:Neuro-vascular communication is essential to synchronize central nervous system development. Here, we identify angiopoietin/Tie2 as a neuro-vascular signaling axis involved in regulating dendritic morphogenesis of Purkinje cells (PCs). We show that in the developing cerebellum Tie2 expression is not restricted to blood vessels, but it is also present in PCs. Its ligands angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2) are expressed in neural cells and endothelial cells (ECs), respectively. PC-specific deletion of Tie2 results in reduced dendritic arborization, which is recapitulated in neural-specific Ang1-knockout and Ang2 full-knockout mice. Mechanistically, RNA sequencing reveals that Tie2-deficient PCs present alterations in gene expression of multiple genes involved in cytoskeleton organization, dendritic formation, growth, and branching. Functionally, mice with deletion of Tie2 in PCs present alterations in PC network functionality. Altogether, our data propose Ang/Tie2 signaling as a mediator of intercellular communication between neural cells, ECs, and PCs, required for proper PC dendritic morphogenesis and function.

| S-EPMC9110807 | biostudies-literature

Gene expression profile in HUVECs before and after Angiopoietin stimulation

2008-04-01 | GSE9677 | GEO

Transcription profiling of human HUVECs before and after Angiopoietin stimulation

Project description:Angiopoietin-Tie2 sytem has been inplicated in both vascular quiescence and angiogenesis. It is unclear how these two opposing signals are regulated from the same receptor-mediated intracellular signal transduction. We have noticed that Tie2 localization upon Angiopoietin stimulation depends upon the presence or absence of cell-cell contacts. Therefore, to identify the downstream signaling of Tie2 upon Angiopoietin stimulation, we performed DNA microarray analyais using RNAs obtained from confluent human umbirical vein endothelial cells (HUVECs) or sparse HUVECs stimulated with after Angiopoietin-1. There is striking difference on gene expression profile between confluent and sparse HUVECs. Experiment Overall Design: To identify the genes in HUVECs which are upregulated or downregulated by the stimulation with Angiopoietin-1 in the presence (confluent) or absence (sparse) of cell-cell contacts, mRNAs were prepared from either confluent HUVECs or sparse HUVECs stimulated or unstimulated with 100 ng/ml COMP-Ang1 (more potent Angiopoietin-1). RNAs were obtained from HUVECs three times cultured independently three times and mixed as one RNA sample for further DNA microarray analyses. Experiment Overall Design: Microarray (Human Genome U133 Plus 2.0 array, Affymetrix) analyses have been performed in duplicate. Biotin labeled probes were used for the series of analyses. Hybridization and Scan were performed according to the recommendation by Affymetric. Data have been processed following the MAS 5.0 with target intensity = 100.

2008-06-16 | E-GEOD-9677 | biostudies-arrayexpress

Single cell analysis in the adult aorta after Tie2 deletion

Project description:To analyze the Angiopoietin receptor Tie2 on the arterial endothelial cells during atheroclerosis

2023-01-23 | GSE161979 | GEO

The angiopoietin receptor Tie2 restricts proliferation and sprouting of cardiac endothelial cells

Project description:The angiopoietin receptor Tie2 restricts proliferation and sprouting of cardiac endothelial cells

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Angiopoietin-Tie2 signaling protects the kidney from ischemia-reperfusion injury in mice

Project description:Angiopoietin-Tie2 signaling protects the kidney from ischemia-reperfusion injury in mice

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The angiopoietin receptor Tie2 restricts proliferation and sprouting of cardiac endothelial cells

Project description:Tie1 and Tie2 receptors are important in the maintenance of the vascular steady-state. We used scRNAseq to analyze how genetic deletion of Tie1 and Tie2 affects expression of proliferation-related genes in mouse endothelial cells.

2026-01-02 | GSE297297 | GEO

Tie2+ vs Tie2- macrophages bulk RNAseq

Project description:Tie2+ vs Tie2- macrophages bulk RNAseq

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Targeting the intersection of angiopoietin-TIE2 signaling with arachidonic acid metabolism can prevent septic death

Project description:Targeting the intersection of angiopoietin-TIE2 signaling with arachidonic acid metabolism can prevent septic death

| PRJNA999296 | ENA