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Transcriptomic study of the HT22 cells after OGD treatment.

ABSTRACT: Transcriptomic study of the HT22 cells after OGD treatment.

PROVIDER: PRJNA741816 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14925900_1.fastq.gz	Fastqsanger.gz
	SRR14925900_2.fastq.gz	Fastqsanger.gz
	SRR14925901_1.fastq.gz	Fastqsanger.gz
	SRR14925901_2.fastq.gz	Fastqsanger.gz
	SRR14925902_1.fastq.gz	Fastqsanger.gz

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Transcriptomic study of the HT22 cells after OGD treatment.

Project description:To investigate the key genes of ischemic stroke, the oxygen glucose deprivation model and RNA sequence of HT22 neuronal cells were performed. By comparing with each other, we identified 51 up-regulated and 119 down-regulated genes in 3H vs 0H, 229 up-regulated and 50 down-regulated genes in 6H-vs-3H, and 145 up-regulated and 121 down-regulated genes in 6H-vs-0H. The results indicate that c-Fos acts as the most key gene at 51 up-regulated genes in 3H vs 0H.

2021-06-28 | GSE178997 | GEO

RNA-seq Analysis of the 5-HT2B Receptor Agonist BW723C86 on OGD-induced HT22 Cells in a Transwell Co-culture System with BMDMs

Project description:Microglia and neurons engage in complex bidirectional communication that is crucial for the response to ischemic injury. To investigate the transcriptional changes underlying this crosstalk, we employed a Transwell co-culture system. Specifically, Bone marrow-derived macrophages（ BMDMs) were co-cultured with HT22 mouse hippocampal neurons subjected to 2 hours of oxygen-glucose deprivation (OGD) to model ischemic conditions. This setup allowed for the paracrine exchange of signals without direct cell contact. Following OGD, the co-culture was treated with either the 5-HT2B receptor agonist BW723C86 or a DMSO vehicle control. We then performed bulk RNA-sequencing on the microglial/BMDM population to profile their transcriptomic response to neuronal injury signals and receptor agonism. Our analysis aims to identify key signaling pathways and gene expression changes in microglia that are modulated by ischemic neurons and the serotonergic system, providing insights into the molecular mechanisms of neuroinflammation and neuroprotection.

2026-07-03 | GSE328636 | GEO

RNA‑seq transcriptomic analysis of HL‑1 cardiomyocytes under OGD/R injury with or without Phillyrin treatment

Project description:Ischemia‑reperfusion (I/R) injury is a key pathological process in myocardial infarction, for which effective therapies are still lacking. Phillyrin has been reported to exert cardioprotective effects, but its molecular mechanisms remain unclear. HL‑1 mouse cardiomyocytes were subjected to oxygen‑glucose deprivation/reoxygenation (OGD/R) to mimic I/R injury. Cells were divided into two groups: OGD/R alone (IR) and OGD/R with Phillyrin treatment (IR+Phil). Total RNA was extracted from 3 biological replicates per group and sequenced using Illumina NovaSeq 6000. Transcriptomic profiling identified differentially expressed genes and pathways regulated by OGD/R and Phillyrin treatment. The processed data file contains raw read counts and FPKM values for all genes across all samples. This dataset provides a comprehensive transcriptomic resource to explore the molecular mechanisms of Phillyrin‑mediated cardioprotection against I/R injury.

2026-05-31 | GSE333299 | GEO

Expression data from J147-treated HT22 cells compared to untreated HT22 cells

Project description:The Neurotrophic Compound J147 Reverses Cognitive Impairment In Aged Alzheimer's Disease We used GeneChipM-BM-. Mouse Genome 430 2.0 Arrays to probe global gene expression changes when adding J147 compound to HT22 cells and compared to untreated cells. The hippocampal cell line HT22 were treated with the J147 compound for 1 hour and compared to HT22 treated only with vehicle (DMSO) for 1 hour. The gene expression profile from the J147-treated HT22 cells (1 hour exposure) was compared to HT22 cells treated with vehicle only (DMSO) for 1 hour. The DMSO/vehicle treated cells serve as the control and are referred to as untreated with drug.

2013-03-28 | E-GEOD-45534 | biostudies-arrayexpress

Expression data from J147-treated HT22 cells compared to untreated HT22 cells

Project description:The Neurotrophic Compound J147 Reverses Cognitive Impairment In Aged Alzheimer's Disease We used GeneChip® Mouse Genome 430 2.0 Arrays to probe global gene expression changes when adding J147 compound to HT22 cells and compared to untreated cells. The hippocampal cell line HT22 were treated with the J147 compound for 1 hour and compared to HT22 treated only with vehicle (DMSO) for 1 hour.

2013-03-28 | GSE45534 | GEO

Global transcriptomic profiling of oxygen-glucose deprivation (OGD)-mediated neuronal injury

Project description:Oxygen-glucose deprivation (OGD) is a cellular phenomenon consistently observed upon occurrence of ischemic stroke which eventually results in neuronal death. Neuronal cell death after ischemia takes place via two distinct processes, necrosis and apoptosis. Apoptosis, programmed cell death, is denoted by chromatin condensation, nuclear blebbing, cellular shrinkage, and DNA fragmentation. Unlike apoptosis, cellular swelling and lysis is suggestive of necrosis. However, these two processes are related not only to the severity but also to the duration of ischemia. Microarray analysis was carried out using 20 Illumina mouse Ref8V1.1 genechip arrays. The assignment of the arrays was as follows: Controls (n=5); exposure to OGD for 10min, 5h, 8h, 15h and 24 h (n=3 respectively).

2013-12-31 | E-GEOD-23158 | biostudies-arrayexpress

The mRNAs alteration induced by Neat1 knockdown in HT22 cells

Project description:We knocked down the lncRNA-Neat1 by RNA interference in HT22 cells, which were immortalized from the mice hippocampal neurons. Then we measured the mRNA alteration induced by Neat1 knockdown through the next generation sequencing technique (RNA-seq). A total of 18,309 transcripts were detected in the current sequencing study, in which 593 transcripts were selected out according to the criteria: |log2(fold change)| > 1, P < 0.05 (22 up-regulated and 571 down-regulated). To explore whether the mRNA change induced by Neat1 knockdown in a stressed culture condition was different from that in normal condition, the cells were dealt with OGD and followed by RNA-seq. A total of 18,502 transcripts were detected, in which 121 transcripts were significantly up-regulated and 126 transcripts were significantly down-regulated according to the aforementioned filter rules.

2019-08-17 | GSE85749 | GEO

RNA‑seq transcriptomic analysis of HL‑1 cardiomyocytes under OGD/R injury with or without Phillyrin treatment

Project description:RNA‑seq transcriptomic analysis of HL‑1 cardiomyocytes under OGD/R injury with or without Phillyrin treatment

| PRJNA1470647 | ENA

The mRNA alteration in HT22 cells induced by the overexpression of long noncoding RNA AK046375

Project description:We overexpressed the lncRNA-AK046375 by lentivirus in HT22 cells, which were immortalized from the mice hippocampal neurons. Then we measured the mRNA alteration induced by AK046375 overexpression through the next generation sequencing technique (RNA-seq). A total of 20,319 transcripts were detected in the current sequencing study, in which 1342 transcripts were selected out according to the criteria: |log2(fold change)| > 1, P < 0.05 (717 up-regulated and 625 down-regulated). To explore whether the mRNA change induced by AK046375 overexpression in a stressed culture condition was different from that in normal condition, the cells were dealt with oxygen-glucose-deprived(OGD) and followed by RNA-seq. A total of 22,016 transcripts were detected, in which 87 transcripts were significantly up-regulated and 131 transcripts were significantly down-regulated according to the aforementioned filter rules.

2020-08-25 | GSE103353 | GEO

Proteomic Profiling of the 5‑HT2B Receptor Agonist BW723C86 on Migration and Signaling in Mouse Bone Marrow‑Derived Macrophages

Project description:Mouse Bone marrow-derived macrophages (BMDMs) and HT22 neuronal cells (subjected to 2-hour oxygen-glucose deprivation, OGD-2h) were used in this study. To investigate cell migration, a Transwell assay was performed. For pharmacological intervention, cells were treated with either the serotonin receptor 2B (5-HT2B) agonist BW723C86 or dimethyl sulfoxide (DMSO) as a vehicle control. Following treatment, global protein expression profiling was performed using mass spectrometry-based quantitative proteomics.

2026-07-04 | PXD074138 | Pride

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