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Camellia sinensis

ABSTRACT: Small RNAs sequence of tea leaves after the infection by Pestalotiopsis trachicarpicola.

PROVIDER: PRJNA742222 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14996693.fastq.gz	Fastqsanger.gz
	SRR14996694.fastq.gz	Fastqsanger.gz
	SRR14996695.fastq.gz	Fastqsanger.gz
	SRR14996696.fastq.gz	Fastqsanger.gz
	SRR14996697.fastq.gz	Fastqsanger.gz

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Project description:Transcriptome analysis of tea leaves after the infection by Pestalotiopsis trachicarpicola.

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Project description:The sequence of small RNAs originating from tea leaves infected or uninfected by Pestalotiopsis trachicarpicola strain ACCC39730

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Project description:The sequence of messenger RNAs (mRNAs) originating from tea leaves infected or uninfected by Pestalotiopsis trachicarpicola strain ACCC39730

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Camellia sinensis strain:ACCC39730

Project description:The sequence of long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) originating from tea leaves infected by Pestalotiopsis trachicarpicola strain ACCC39730.

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Characterizing microRNAs and their targets in differnt organs of Camellia sinensis [degradome]

Project description:MicroRNAs (miRNAs) are a type of small non-coding RNAs, which play important roles in plant growth, development and stress responses. Tea (Camellia sinensis) prepared from tea tree is the oldest and most popular nonalcoholic beverages in the world, and has large economic, medicinal and cultural significance. Nevertheless, there are a few studies on the miRNAs and their functions in Camellia sinensis. We sequenced 9 small RNA libraries and 9 RNA-Seq libraries from roots, leaves and flowers tissues. Through comprehensive computational analyses of 9 small RNA profiles, we identified 200 conserved miRNAs of which 138 have not been reported, and 56 novel miRNAs with 33 have not been reported. Nearly, two thousands genes have significantly different expression levels in tissues. In order to identify targets of miRNAs, we sequenced two degradome profiles from leaves and roots, respectively. Totally, more than 3,000 putative targets of conserved miRNAs were identified in both degradome profiles by using the SeqTar algorithm. These results clearly enhanced our understanding about small RNA guided gene regulations in Camellia sinensis.

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Characterizing microRNAs and their targets in differnt organs of Camellia sinensis [miRNA-seq]

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Identification and characterization of cold responsive microRNAs in tea plant (Camellia sinensis) based on high-throughput sequencing and their targets using degradome analysis [microarray]

Project description:Solexa sequencing technology was used to perform high throughput sequencing of the small RNA library from the cold treatment of tea leaves. Subsequently, aligning these sequencing date with plant known miRNAs, we characterized 112 C. sinensis conserved miRNAs. In addition, 215 potential candidate miRNAs were found; among them, 131 candidates with star sequence were chosen as novel miRNAs. There are both congruously and differently regulated miRNAs, and line-specific miRNAs were identified by microarray-based hybridization in response to cold stress. The miRNA chip included 3228 miRNA probes corresponding to miRNA transcripts listed in Sanger miRBase release 19.0 and 283 novel miRNAs probes founding in tea plant. In the study presented here, two tea plant cultivars, ‘Yingshuang’ (YS, a cold-tolerant tea plant cultivar) and ‘Baiye 1’ (BY, a cold-sensitive tea plant cultivar), were kept at 4°C for 4,12, 24 h, respectively, and 28°C for as control. These samples were used to acquire expression profiles of a total of 3,511 unique genes, leading to the successful construction of supervised

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Identification and characterization of cold responsive microRNAs in tea plant (Camellia sinensis) based on high-throughput sequencing and their targets using degradome analysis [microarray]

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