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ABSTRACT: Differential expression genes in Arabidopsis under flg22 treatment

PROVIDER: PRJNA742510 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15015667_1.fastq.gz	Fastqsanger.gz
	SRR15015667_2.fastq.gz	Fastqsanger.gz
	SRR15015668_1.fastq.gz	Fastqsanger.gz
	SRR15015668_2.fastq.gz	Fastqsanger.gz
	SRR15015669_1.fastq.gz	Fastqsanger.gz

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Expression analysis of Arabidopsis mpk11 mutant treated with flg22

Project description:Arabidopsis MPK11 is activated and plays a role in the flg22 sensing. Mitogen-activated protein kinases (MAPKs) mediate cellular signal transduction during stress responses, as well as diverse growth and developmental steps in eukaryotes. Pathogen infection or treatment with conserved pathogen-associated molecular patterns (PAMPs) such as the bacterial flagellin-derived flg22 peptide are known to activate three Arabidopsis thaliana MAPKs, MPK3, MPK4 and MPK6. Several stresses, including flg22 treatment, are known to increase MPK11 expression but activation of MPK11 has not been shown. Here, we show that MPK11 activity can indeed be increased through flg22 elicitation. Expression profiling using a small-scale microarray for defense-related genes revealed that cinnamyl alcohol dehyrogenase 5 (CAD5) requires MPK11 for full flg22-induced expression. An mpk11 mutant showed increased flg22-mediated growth inhibition but no altered susceptibility to Pseudomonas syringae, Botrytis cinerea or Alternaria brassicicola.

2012-05-03 | GSE33648 | GEO

Expression analysis of Arabidopsis mpk11 mutant treated with flg22

2012-05-02 | E-GEOD-33648 | biostudies-arrayexpress

Plasma membrane proteomics of Arabidopsis responses to flagellin

Project description:Arabidopsis plasma membrane-enriched fractions were profiled 180 and 720 minutes after treatment with water or flg22 peptide.

2016-04-06 | MSV000079639 | MassIVE

RNAseq analysis of the transcriptome of an Arabidopsis mutant, ahl13, elicitated by pstDC3000 or flg22

Project description:Purpose: The experiment is to compare the NGS-derived seedling and leaf transcriptome profiling (RNAseq) of an Arabidopsis mutant ahl13 with that of wild type upon bacterial pathogen (pst DC3000) and flagellin elicitation. Method： The mRNA profiles of 10-day-old wild-type (WT) and AHL13 knockout (ahl13, SALK_014014) Arabidopsis were generated by RNAseq using Illumina NovaSeq6000. The sequence reads were passed through quality filters and mapped to Arabidopsis genome by TopHat2 followed by transcript splicing and quantitation by Cufflinks. DESeq was done to analyze differential gene expressions. qRT–PCR validation was performed using SYBR Green assays Result: We mapped about 20 million sequencing reads per sample to the Arabidopsis genome and identified approximately 28,000 transcripts in the Arabidopsis Col-0 and ahl13 mutant using TopHat2. Differentially expressed genes were identified using DESeq2 and about 166 genes show up-regulation in ahl13 upon DC3000 infection (FC>2, FDR<0.01) Conclusions: The ahl13 mutant exhibit differential transcription profile from that of Col-0 upon DC3000 treatment but similar upon flg22 treatment.

2022-01-13 | GSE193345 | GEO

Genome-wide maps of AGO1-bound small RNA in Arabidopsis leaves

Project description:We report flg22 regulate the accumulation of AGO1-bound small RNA in arabidopsis. We find that a number of miRNAs are up- or down-regulated by flg22, a well-studied PAMP.

2010-02-25 | GSE20448 | GEO

Genome-wide maps of AGO1-bound small RNA in Arabidopsis leaves

Project description:We report flg22 regulate the accumulation of AGO1-bound small RNA in arabidopsis. We find that a number of miRNAs are up- or down-regulated by flg22, a well-studied PAMP. Examination of AGO1-bound small RNAs with or without flg22 treatment.

2010-02-25 | E-GEOD-20448 | biostudies-arrayexpress

Transcriptome Analysis of Arabidopsis thaliana and Changes of Glucosinolates Metabolism Pathway Induced by Flg22

Project description:Purpose: The goals of this study are to analyze the transcriptome of Arabidopsis thaliana treated after flg22 and to find changes of glucosinolate metabolism genes after treatment. Methods: Total mRNA of 10 day wild-type Arabidopsis seedlings that were treated with and without flg22( final concentrations 1μmol/L) in 1/2 MS medium for 4h, were extracted respectively. Each sample was harvested in three independent biological replicates with equal weight and mixed ,subsequently sequencing. The sequence reads that passed quality filters were mapped to the Arabidopsis_thaliana genome (TAIR10.18).Then the mapping genes were used for the abundance and functional analysis. Results: We mapped about 45 million and 52 million sequence reads of control sample and treatment sample to the Arabidopsis_thaliana genome (TAIR10.18) and identified total 23,413 genes with Botiw/TopHat workflow. Comparison of the two samples showed 1,200 differentially expressed genes (DEGs), including 290 down-regulated and 910 up-regulated genes. The DEGs were associated with energy metabolism, amino acid metabolism and biosynthesis of secondary metabolites. After flg22 treatment, genes involved in indolic glucosinolate biosynthesis pathway were up-regulated significantly，which is further demonstrated by Real Time RT-PCR, while aliphatic glucosinolate pathway almost had no change, indicating the important role of indolic glucosinolates in plant defense responses. Conclusion: Our study provides the overall genetic resource of Arabidopsis_thaliana after treated by flg22 to date. These data will pave the way for further studies about deeply understand pathogen induced defense and the contribution of indolic glucosinolates.

2017-03-22 | GSE56117 | GEO

Phosphoproteomics of Arabidopsis flg22 treated nuclear samples

Project description:To study the effect of microbe-associated molecular pattern (MAMP) treatment (flg22) on the phosphorylation of nuclear proteins, we treated Arabidopsis plants with flg22 and after isolation of nuclear proteins, we enriched for phosphopeptides and then carried out LC-MS/MS analyses. We used statistical analysis tools to identify differentially phosphorylated proteins in response to MAMP treatment in the mock and treated samples.

2022-02-28 | PXD008403 | Pride

Gene expression changes underlying initial cellular and molecular responses to MAMPs in Arabidopsis mesophyll cells

Project description:This SuperSeries is composed of the following subset Series: GSE16471: Identification of CPK5ac and CPK11ac early target genes in Arabidopsis mesophyll cells GSE16472: Identification of early flg22 responsive genes in Arabidopsis mesophyll cells GSE17382: Identification of early flg22 responsive genes in Arabidopsis seedlings Refer to individual Series

2010-03-18 | E-GEOD-16557 | biostudies-arrayexpress

Flg22 treatment of Brassicaceae and Arabidopsis thaliana accessions

Project description:Flg22 treatment of Brassicaceae and Arabidopsis thaliana accessions

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