Ontology highlight
ABSTRACT:
PROVIDER: PRJNA742547 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR15020100_1.fastq.gz | Fastqsanger.gz | |||
| SRR15020100_2.fastq.gz | Fastqsanger.gz | |||
| SRR15020101_1.fastq.gz | Fastqsanger.gz | |||
| SRR15020101_2.fastq.gz | Fastqsanger.gz | |||
| SRR15020092_1.fastq.gz | Fastqsanger.gz |
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Project description:The algal holobiont, which consists of algae and the bacteria living on it, has developed strategies to handle environmental stresses, such as changes in temperature. The green alga Ulva (Chlorophyta) is home to bacteria that help the algae grow and develop, from germlings to mature plants. These bacteria release chemicals like thallusin, N-acyl homoserine lactone, and ectoine, which support the health and growth of the algae. However, there is limited research on the metabolome of cold-adapted Ulva holobionts, especially those from hard-to-reach regions like Antarctica, and how they respond to environmental changes. In this study, we examine the metabolites found in Ulva and its associated bacteria from Antarctica (Potter Cove) using high-resolution mass spectrometry. We identified small polar and zwitterionic metabolites, such as cysteinolic acid, ectoine, glutamine, glycerol, and proline, in the surface tissues of the algae with MALDI-MS. By profiling these metabolites using UHPLC-HRMS and ZIC-HILIC chromatography, we found that their levels change significantly in response to heat-stressed Antarctic Ulva holobionts. Our results suggest that environmental change can affect the interaction between seaweeds and their microbes by altering the metabolites involved in their chemical exchanges.
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2025-03-06 | MSV000097253 | MassIVE
Project description:Bacterial communities associated with the marine green algae from the genera Ulva
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Project description:We recently reported that the in vitro and in vivo survival of Rickettsia australis are Atg5-dependent, in association with an inhibited level of anti-rickettsial cytokine, IL-1β. In the present study, we sought to investigate how R. australis interacts with host innate immunity via an Atg5-dependent autophagic response. We found that the serum levels of IFN-γ and G-CSF in R. australis-infected Atg5flox/floxLyz-Cre mice were significantly less compared to Atg5flox/flox mice, accompanied by significantly lower rickettsial loads in tissues with inflammatory cellular infiltrations including neutrophils. R. australis infection differentially regulated a significant number of genes in bone marrow-derived macrophages (BMMs) in an Atg5-depdent fashion as determined by RNA sequencing and Ingenuity Pathway Analysis, including genes in the molecular networks of IL-1 family cytokines and PI3K-Akt-mTOR. The secretion levels of inflammatory cytokines, such as IL-1α, IL-18, TNF-α, and IL-6, by R. australis-infected Atg5flox/floxLyz-Cre BMMs were significantly greater compared to infected Atg5flox/flox BMMs. Interestingly, R. australis significantly increased the levels of phosphorylated mTOR and P70S6K at a time when the autophagic response is induced. Rapamycin treatment nearly abolished the phosphorylated mTOR and P70S6K but did not promote significant autophagic flux during R. australis infection. These results highlight that R. australis modulates an Atg5-dependent autophagic response, which is not sensitive to regulation by mTORC1 signaling in macrophages. Overall, we demonstrate that R. australis counteracts host innate immunity including IL-1β-dependent inflammatory response to support the bacterial survival via an mTORC1-resistant autophagic response in macrophages.
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Project description:Ulva sp., a promising yet underexplored food source, offers rich protein content and nutritional benefits. However, assessing its allergenicity remains challenging due to limited research and understanding. In this translational research, we focus on a laboratory-produced Ulva sp. product (UlvaLe) and gather comprehensive data to assess its potential allergenicity. Therefore, we employed a weight-of-evidence approach inspired by regulatory standards, conducting a literature review, proteomic analysis, and clinical trial. This article addresses challenges in assessing the allergenicity of novel foods, using Ulva sp. as an example, while enhancing the understanding of Ulva sp.'s allergenic potential and offers insights for future research and regulatory processes. Our findings reveal no reported cases of Ulva allergenicity in the literature but highlight the need to address polysaccharide safety. Proteins with homology exceeding 70% to known allergens were identified as putative allergens. Nonetheless, interim clinical trial results demonstrate no allergic reactions to UlvaLe ingestion, suggesting a favorable safety profile. The presented data suggests a promising allergenicity safety profile for UlvaLe as a novel food source. Further research into macro-algae allergenicity, including DNA and protein composition decoding, is warranted to ensure safe utilization in the food industry. Nevertheless, the most effective means of assessing allergenicity for any new food is through widespread consumption among large populations, which statistically reveals the true public risk. As a modern society seeking innovative solutions and sustainable growth, it is imperative to be mindful of this and devise strategies for its safe integration.
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