Project description:Transcription profiling by array of pancreatic tissues from wild-type mice, Mist1 knockout mice and Mist1 knockout mice with induced (rescued) MIST1 expression
Project description:The physiological functions other than lipid metabolism of APOE may interfere with the research results of Apoe-knockout mice as atherosclerosis models. The elucidation of the molecular basis of the physiological functions of APOE other than lipid metabolism will help to make rational use of the Apoe-knockout mouse model. Therefore, our aim is to reveal the changes in aortic gene expression profile caused by Apoe-knockout. We used the second generation sequencing to detect the gene mRNA expression level in the aortic tissues of 5 C57BL/6J mice and 4 Apoe knockout mice. This will provide more clues for studying the physiological function of Apoe, and also remind researchers to pay special attention to the possible interference to the results when using Apoe-knockout mice as atherosclerosis models.
2024-10-31 | GSE215969 | GEO
Project description:Gut Microbiome in ApoE knockout mice
Project description:ApoE-/-mice were fed chow or Western diet for 12 weeks and NPRC expression was significantly increased in the aortic tissues of Western diet-fed mice. Systemic NPRC knockout mice were crossed with ApoE-/- mice to generate ApoE-/-NPRC-/- mice, and NPRC deletion resulted in a significant decrease in the size and instability of aortic atherosclerotic lesions in ApoE-/-NPRC-/- versus ApoE-/- mice.