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Temporal response of Mycobacterium tuberculosis H37Rv to vitamin C exposure in THP-1 macrophages.

ABSTRACT: Temporal response of Mycobacterium tuberculosis H37Rv to vitamin C exposure in THP-1 macrophages.

PROVIDER: PRJNA743509 | ENA |

REPOSITORIES: ENA

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Temporal response of Mycobacterium tuberculosis H37Rv to vitamin C exposure in THP-1 macrophages.

Project description:Transcriptional profiling of Mtb H37Rv infected into THP-1 macrophage cell line and treated with 100 µM vitamin C (vit C) for 96 hours and 144 hours, compared to gene expression profile of untreated bacteria post-infection.

2021-10-01 | GSE179376 | GEO

Temporal response of Mycobacterium tuberculosis H37Rv to vitamin C exposure.

Project description:Transcriptional profiling of M.tuberculosis to 10 mM vitamin C at 0.25, 0.5, 1, 2 , 4, 8 and 24 h compared to gene expression profile of untreated M. tuberculosis culture (0 h).

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Transcriptomics of vitamin D treatment effects in Mycobacterium tuberculosis-infected THP-1 cells

Project description:Previous reports have shown low vitamin D serum levels and polymorphisms in the vitamin D receptor (VDR) to be associated with increased risk for TB. Given that 1?,25-dihydroxyvitamin D3 has a role in lipid metabolism control, we tested whether the link between 1?,25-dihydroxyvitamin D3 and tuberculosis involves macrophage lipid metabolism. Since formation of lipid droplets (LD) is a hallmark of lipid dysregulation in M. tuberculosis-infected macrophages, we measured LD content as a readout of altered lipid metabolism in infected THP-1 cells. Induction of LD, which peaked by 24 hours post-infection was prevented by addition of 1?,25-dihydroxyvitamin D3 at the time of infection. To investigate the mechanism of 1?,25-dihydroxyvitamin D3 modulation of LD formation, we analyzed the transcriptome of M. tuberculosis-infected THP-1 cells with and without 1?,25-dihydroxyvitamin D3 treatment. THP-1 cells were cultured in 24-well flat bottom plates, pre-treated with 20ng/ml PMA for 24 h, and incubated with M. tuberculosis H37Rv. After 4 hours of infection, cells were washed to remove extracellular bacteria and treated with 100 nM of 1?,25-dihydroxyvitamin D3. THP-1 cells were collected and analyzed at 24h. 3 biological replicates in each of the 4 groups were prepared: Infected, Infected plus 1?,25-dihydroxyvitamin D3, Non-infected, Non-infected plus 1?,25-dihydroxyvitamin D3.

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The association and difference of normal THP-1 cell and H37Rv infected THP-1 cell.

Project description:①Background:Tuberculosis is mainly a respiratory tract infection caused by mycobacterium tuberculosis and one of the leading causes of death worldwide. According to the Global Tuberculosis Report in 2021, About a quarter of the world's population is infected with Mycobacterium tuberculosis and China is the second highest burden of TB. Although TB diagnosis and prevention techniques have become more mature, the number of TB cases is still increasing, mainly due to: the prevalence of drug-resistant tuberculosis bacteria, tuberculosis and HIV co-infection, long incubation time of mycobacterium tuberculosis difficult to early diagnosis and so on. Therefore, it is of great significance to study the pathogenesis of mycobacterium tuberculosis infection.②Method: THP-1 cells were treated with 50ng/ml PMA for 24 hours, so that THP-1 cell can be induced into macrophages. After that THP-1 macrophages were infected with mycobacterium tuberculosis H37Rv(MOI=1), which were collected and applied to RNA-sequencing. The constructed sequencing library was sequenced using an Illumina Novaseq 6000 system.

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Response of M. tuberculosis to 8 hour exposure to vitamin C

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THP-1 cells infected with H37Rv

Project description:Cells were seeded in 24-well plates with phorbol myristate (PMA, Sigma Aldrich, USA) at a concentration of 100 ng/ml. Adherent THP-1 cells were infected with H37Rv at an MOI of 1 for 4 hours, and then washed three times with PBS to remove bacteria. Experiments involving live H37Rv were performed in a P2+ facility.

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Identification of genes differentially regulated upon infection of Thp-1 cells with H37Rv

Project description:Perturbation of host physiology by intracellular Mycobacterium tuberculosis (H37Rv) can be reflected by changes in gene expression pattern of host genes. Total RNA was isolated from PMA differentiated uninfected cells or cells infected with H37Rv for 16, 48 or 90 hours and gene expression profile was obtained. There are in all 8 samples, two replcates of each 4. Two samples, namely 1D,1E (replicates of one) are control (PMA differentiated Thp-1 cells Uninfected controls). Cells were infected with H37Rv at an MOI of 1:10 and samples were collected at 16 hours, 48 hours and 90 hours post-infection.

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Project description:Temporal response of Mycobacterium tuberculosis H37Rv to vitamin C exposure.

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THP-1 cells infected with H37Rv

Project description:THP-1 cells infected with H37Rv

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