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Transcriptome of miR-290-295 KO mESCs

ABSTRACT: Transcriptome of miR-290-295 KO mESCs

PROVIDER: PRJNA743870 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15043350.fastq.gz	Fastqsanger.gz
	SRR15043351.fastq.gz	Fastqsanger.gz
	SRR15043352.fastq.gz	Fastqsanger.gz
	SRR15043353.fastq.gz	Fastqsanger.gz

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Transcriptome of miR-290-295 KO mESCs

2022-07-15 | GSE179450 | GEO

Transcriptomes of miR-290-295 KO mESCs treated with siPOOLs against the transcription factor Tfap4

Project description:In order to identify direct microRNA targets in mouse embryonic stem cells (mESCs), we knocked out microRNA effector genes, profiled their transcriptomes and integrated the data with additional published OMICs data sets. To validate the analysis, we generated miR-290-295 KO mutant cell lines, assessed the transcriptome profile and compared the observed misregulation to the predictions of the analysis. We identified the transcription factor Tfap4 as novel potential direct and functional target of miR-290-295. To assess the role of Tfap4, we rescued its expression in the miR-290-295 KO cell line using siPOOLs and profiled the transcriptome.

2022-07-15 | GSE181393 | GEO

Transcriptomes of miR-290-295 KO mESCs treated with siPOOLs for three different transcription factors and a negative control

Project description:In order to identify direct microRNA targets in mouse embryonic stem cells (mESCs), we generated miR-290-295 KO mutant cell lines, assessed their transcriptomes. We identified novel transcription factors (TF) as potential direct and functional targets of miR-290-295. To assess the role of these TFs, we rescued their expression in the miR-290-295 KO cell line using siPOOLs and profiled their transcriptomes.

2022-07-15 | GSE179451 | GEO

Transcriptomes of miR-290-295 KO mESCs treated with siPOOLs against the transcription factor Tfap4

Project description:Transcriptomes of miR-290-295 KO mESCs treated with siPOOLs against the transcription factor Tfap4

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A latent pro-survival function for the mir-290-295 cluster in mouse embryonic stem cells.

Project description:MicroRNAs (miRNAs) post-transcriptionally regulate the expression of thousands of distinct mRNAs. While some regulatory interactions help to maintain basal cellular functions, others are likely relevant in more specific settings, such as response to stress. Here we describe such a role for the mir-290-295 cluster, the dominant miRNA cluster in mouse embryonic stem cells (mESCs). Examination of a target list generated from bioinformatic prediction, as well as expression data following miRNA loss, revealed strong enrichment for apoptotic regulators, two of which we validated directly: Caspase 2, the most highly conserved mammalian caspase, and Ei24, a p53 transcriptional target. Consistent with these predictions, mESCs lacking miRNAs were more likely to initiate apoptosis following genotoxic exposure to gamma irradiation or doxorubicin. Knockdown of either candidate partially rescued this pro-apoptotic phenotype, as did transfection of members of the mir-290-295 cluster. These findings were recapitulated in a specific mir-290-295 deletion line, confirming that they reflect miRNA functions at physiological levels. In contrast to the basal regulatory roles previously identified, the pro-survival phenotype shown here may be most relevant to stressful gestations, where pro-oxidant metabolic states induce DNA damage. Similarly, this cluster may mediate chemotherapeutic resistance in a neoplastic context, making it a useful clinical target.

2011-06-18 | GSE30012 | GEO

A latent pro-survival function for the mir-290-295 cluster in mouse embryonic stem cells.

2011-06-18 | E-GEOD-30012 | biostudies-arrayexpress

Transcriptomes of miR-290-295 KO mESCs treated with siPOOLs for three different transcription factors and a negative control

Project description:Transcriptomes of miR-290-295 KO mESCs treated with siPOOLs for three different transcription factors and a negative control

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Expression profiling in miR-290 6dt1 mammary tumors

Project description:mRNA expression data from mammary tumors extracted 30 days after orthotopic injection of miR-290-expressing and negative control 6dt1 cells into female FVB/N mice. At 6 weeks of age, FVB/N female mice were orthotopically injected with 1.0x10E5 6dt1 cells, either expressing miR-290 or a negative control RNA construct. 4 negative control tumors and 3 miR-290 tumors were analyzed.

2013-01-15 | E-GEOD-43485 | biostudies-arrayexpress

Pluripotency-associated miR-290/302 family of microRNAs promote the dismantling of naive pluripotency

Project description:Upon RNA profiling of WT and Dgcr8-/- mESCs in 2iL and differentiation for 48 hours, miRNAs are proved to be important for the transcriptional changes during ESC exiting from pluripotency. Further miRNA sequencing of WT ESCs in different timepoints revealed that miRNAs expression changes dynamically during ESC to EpiLC transition and identified miR-290/302 as key miRNAs facilitating the dismantling of naive pluripotency.

2018-11-29 | GSE123096 | GEO

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Project description:A family of vertebrate-specific microRNAs called the ESCC microRNAs regulates proliferation and differentiation of embryonic stem cells. The ESCC microRNAs arise from two genetic loci in mammals, the miR-290/miR-371 and miR-302 loci. While the miR-302 locus is found broadly among vertebrates, the miR-290/miR-371 locus is unique to eutherian species, suggesting a role in placental development. Here, we evaluate that role. A knockin reporter for the mouse miR-290 gene is expressed throughout the embryo until gastrulation, at which time it becomes specifically expressed in extraembryonic tissues and the germline. In the placenta, expression is limited to the trophoblast lineage, where it remains highly expressed until birth. Deletion of the miR-290 gene results in reduced trophoblast progenitor cell proliferation and a reduced DNA content in endoreduplicating trophoblast giant cells. A reduction in placental size precedes a reduction in fetal size and prenatal death of most knockout embryos. The vascular labyrinth shows disorganization with thickening of the barrier between maternal and fetal blood associated with reduced diffusion of a radioactive tracer. Multiple mRNA targets of the cluster miRNAs are upregulated. Together, these data uncover a critical function for the miR-290 in the regulation of a network of genes required for normal placental development, suggesting a central role for this microRNA cluster in the evolution of eutherian species.

2017-03-04 | GSE95687 | GEO

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