Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Two-step regulation of centromere distribution by condensin II with LINC complex and nuclear lamina

ABSTRACT: Two-step regulation of centromere distribution by condensin II with LINC complex and nuclear lamina

PROVIDER: PRJNA743903 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Two-step regulation of centromere distribution by condensin II with LINC complex and nuclear lamina [36 samples]

Project description:Two-step regulation of centromere distribution by condensin II with LINC complex and nuclear lamina [36 samples]

| PRJNA744997 | ENA

Two-step regulation of centromere distribution by condensin II with LINC complex and nuclear lamina

Project description:To identify genes affected by the mutations in CAP-H2 and CRWN1/4.

2022-06-15 | GSE179466 | GEO

Two-step regulation of centromere distribution by condensin II with LINC complex and nuclear lamina [36 samples]

Project description:To identify genes affected by the mutations in CAP-H2 and CRWN1/4 under the DNA damaging condition

2022-06-15 | GSE179793 | GEO

M18BP1 is a determinant of Condensin II activation

Project description:Condensin I and Condensin II promote drastic spatial compaction (condensation) of the genome upon mitotic entry. Condensin I binds chromatin only after dissolution of the nuclear envelope in prophase. Conversely, Condensin II is nuclear throughout the cell cycle and initiates chromosome condensation. Previous studies demonstrated that MCPH1 inhibits Condensin II to prevent chromosome condensation during interphase. Mechanisms that promote Condensin II activation in early mitosis likely exist but are currently unknown. Through genetic and proteomic approaches, we identify M18BP1, a protein previously associated with centromere identity, as a factor required for Condensin II localization to chromatin during mitosis. To activate and localize Condensin II, M18BP1 directly binds the CAP-G2 subunit. M18BP1 and MCPH1 compete for CAP-G2 binding, and CDK1 phosphorylation promotes replacement of MCPH1 with M18BP1 to activate Condensin II upon mitotic entry. Our results identify a fundamental and evolutionarily conserved mechanism of Condensin II activation

2025-11-03 | PXD051556 | Pride

Condensin II Activation by the centromeric protein M18BP1

Project description:Condensin I and condensin II promote drastic spatial compaction (condensation) of the genome upon mitotic entry. Condensin I binds chromatin only after dissolution of the nuclear envelope in prophase. Conversely, condensin II is nuclear throughout the cell cycle and initiates chromosome condensation. Previous studies demonstrated that MCPH1 inhibits condensin II to prevent chromosome condensation during interphase. Mechanisms that promote condensin II activation in early mitosis likely exist but are currently unknown. Through genetic and proteomic approaches, we identify M18BP1, a protein previously associated with centromere identity, as a factor required for condensin II localization to chromatin during mitosis. To activate and localize condensin II, M18BP1 directly binds the CAP-G2 subunit. M18BP1 and MCPH1 compete for CAP-G2 binding, and CDK1 phosphorylation promotes replacement of MCPH1 with M18BP1 to activate condensin II upon mitotic entry. Our results identify a fundamental and evolutionarily conserved mechanism of condensin II activation.

2025-07-09 | PXD051886 | Pride

Condensin IDC, H4K20me1, and perinuclear tethering maintain X chromosome repression in C. elegans

Project description:Dosage compensation in Caenorhabditis elegans equalizes X-linked gene expression between XX hermaphrodites and XO males. The process depends on a condensin-containing dosage compensation complex (DCC), which binds the X chromosomes in hermaphrodites to repress gene expression. Condensin IDC and an additional five DCC components must be present on the X during early embryogenesis in hermaphrodites to establish dosage compensation. However, whether the DCC's continued presence is required to maintain the repressed state once established is unknown. Beyond the role of condensin IDC in X chromosome compaction, additional mechanisms contribute to X-linked gene repression. DPY-21, a non-condensin IDC DCC component, is an H4K20me2/3 demethylase whose activity enriches the repressive histone mark, H4 lysine 20 monomethylation on the X chromosomes. In addition, CEC-4 tethers H3K9me3-rich chromosomal regions to the nuclear lamina, which also contributes to X-linked gene repression. To investigate the necessity of condensin IDC during the larval and adult stages of hermaphrodites, we used the auxin-inducible degradation system to deplete the condensin IDC subunit DPY-27. While DPY-27 depletion in the embryonic stages resulted in lethality, DPY-27 depleted larvae and adults survive. In these DPY-27 depleted strains, condensin IDC was no longer associated with the X chromosome, the X became decondensed, and the H4K20me1 mark was gradually lost, leading to X-linked gene derepression. These results suggest that the stable maintenance of dosage compensation requires the continued presence of condensin IDC. A loss-of-function mutation in cec-4, in addition to the depletion of DPY-27 or the genetic mutation of dpy-21, led to even more significant increases in X-linked gene expression, suggesting that tethering heterochromatic regions to the nuclear lamina helps stabilize repression mediated by condensin IDC and H4K20me1.

2025-01-25 | GSE262451 | GEO

Linc-ADAIN Pulldown assay in Human Adipocytes.

Project description:Description: a biotinylated pulldown assay of linc-ADAIN, lincRNA vs Antisense Linc-ADAIN in primary human ASC adipocytes coupled to mass spectrometry (MS).

2024-10-17 | PXD051388 | Pride

LINC-JP_PCAWG_WGS_BWA - samples

Project description:ICGC PCAWG Dataset for WGS BAM aligned using BWA MEM. Project: LINC-JP.

| EGAD00001002662 | EGA

Step

Project description:Step Raw sequence reads

| PRJNA841025 | ENA

Acute condensin depletion causes genome decompaction without altering the level of global gene expression in Saccharomyces cerevisiae

Project description:Condensins are broadly conserved chromosome organizers that function in chromatin compaction and transcriptional regulation, but to what extent these two functions are linked has remained unclear. Here, we analyzed the effect of condensin inactivation on genome compaction and global gene expression in the yeast Saccharomyces cerevisiae. Spike-in-controlled 3C-seq analysis revealed that acute condensin inactivation led to a global decrease in close-range chromosomal interactions as well as more specific losses of homotypic tRNA gene clustering. In addition, we identified a condensin-rich topologically associated domain between the ribosomal DNA and the centromere on chromosome XII that is lost upon condensin inactivation. Unexpectedly, these large-scale changes in chromosome architecture were not associated with global changes in transcript levels as determined by spike-in-controlled mRNA-seq analysis. Our data suggest that the global transcriptional program of S. cerevisiae is resistant to condensin inactivation and the associated profound changes in genome organization.

2017-10-25 | GSE106104 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data