Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Whole transcriptome sequencing of Npas3+/- and WT cortex at P0 stage

ABSTRACT: Whole transcriptome sequencing of Npas3+/- and WT cortex at P0 stage

PROVIDER: PRJNA744260 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15054752_1.fastq.gz	Fastqsanger.gz
	SRR15054752_2.fastq.gz	Fastqsanger.gz
	SRR15054753_1.fastq.gz	Fastqsanger.gz
	SRR15054753_2.fastq.gz	Fastqsanger.gz
	SRR15054754_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Whole transcriptome sequencing of Npas3+/- and WT cortex at P0 stage

Project description:Purpose: To gain insight into the impact of NPAS3 deficiency on astrogenesis in mice, RNA-seq was used to analyze the genome-wide changes resulting from Npas3+/- and WT cortex at P0 stage. Methods: Total RNA was extracted from Npas3+/- and WT cortex at P0 stage. Then, total RNA was quantified and quality controlled by Agilent Bioanalyzer 2100 system. After cDNA library construction, the library preparations were sequenced on an Illumina NovaSeq 6000 platform in Novogene. Results: At significance cutoffs corresponding to FDR < 0.05, a total of 625 DEGs were identified in Npas3+/- mice. Conclusions: Our results show that Npas3+/- mice exhibit defective astrogenesis and abnormal synapse function.

2022-07-19 | GSE179608 | GEO

RNA-sequencing experiments comparing P0 control and RXRG/RARb double mutants frontal cortex

Project description:To understand the functional significance of RA signaling through the RXRG/RARB heterodimer in the developing cortex, we performed RNA-seq experiments comparing different regions of the P0 mouse frontal cortex (mPFC, secondary motor cortex (MOs)/ primary motor cortex (MOp), and OFC ) in Rarb and Rxrg dKO and WT littermate controls.

2021-01-01 | GSE142851 | GEO

RNA-seq analysis of WT vs. Reck vascular KO P0 mouse brains

Project description:We examined, compared, and analyzed the transcriptome profile (RNA-seq) of WT vs Reck vascular KO (Reck flex2/Δex1; Tie2Cre) P0 mouse brains. Specifically, cortical transcriptomic differences (between WT and mutant genotypes) were compared separately from subcortical transcriptomic changes.

2017-08-10 | GSE94814 | GEO

mRNA sequencing of P0 primary WT and Mettl3/14nKO mouse islets

Project description:Using RNA-seq, we identified 945 differentially expressed genes in P0 KO islets, compared to WT（fc>1.5）. Among them, a cluster of genes involved in insulin secretion, ion transportation were significantly changed.

2020-04-24 | GSE149193 | GEO

Transcriptomic analysis of the cortex of ASPM Knockout Macaca fascicularis at P0

Project description:Transcriptomic analysis of the cortex of ASPM Knockout Macaca fascicularis at P0

| PRJNA526884 | ENA

Whole esophagi of WT, Nrf2-/- and Keap1-/- mice on C57BL background at E11.5, E15.5, P0 and P7, and esophageal epithelium of adult (8 weeks old) mice

Project description:Transcriptional profiling of mouse esophageal development. Goal was to globally profile critical genes and signaling pathways during the development of mouse esophagus and determine how Nrf2/Keap1 pathway regulates the morphogenesis of the esophageal epithelium. Mutiple-comparison. WT-E11.5 vs. WT-E15.5 vs. WT-P0 vs. WT-P7; WT-P7 vs. WT-adult; WT-adult vs. Nrf2-/--adult; WT-P7 vs. Nrf2-/--P7 vs. Keap1-/--P7 vs. Nrf2-/-Keap1-/--P7. Biological replicates: 3 replicates for each group.

2011-12-09 | E-GEOD-34278 | biostudies-arrayexpress

mRNA sequencing of P0 primary WT and Mettl3/14nKO mouse islets

Project description:mRNA sequencing of P0 primary WT and Mettl3/14nKO mouse islets

| PRJNA627643 | ENA

Whole transcriptome sequencing of mouse notochord-derived cells at developmental stages E12.5 and P0

Project description:Recapitulation of developmental signals represents a promising strategy for treating intervertebral disc degeneration. During development, embryonic notochord-derived cells (NDCs) are the direct progenitors of cells that populate the adult nucleus pulposus (NP) and are an important source of secreted signaling molecules. The objective of this study was to define global gene expression profiles of NDCs at key stages of embryonic disc formation. NDCs were isolated from Shh-cre;ROSA:YFP mice at embryonic day 12.5 and postnatal day 0, representing opposite ends of the notochord to NP transformation. Differences in global mRNA abundance across this developmental window were established using RNA-Seq. Principal component analysis revealed clustering of gene expression at each developmental stage with more than 5000 genes significantly differentially expressed between E12.5 and P0. This study represents the first transcriptome-wide analysis of embryonic NDCs. Results suggest signaling and biosynthesis of NDCs change dramatically as a function of developmental stage.

2017-09-05 | GSE100934 | GEO

RNA-sequencing experiments comparing P0 control and RXRG/RARb double mutants frontal cortex

Project description:RNA-sequencing experiments comparing P0 control and RXRG/RARb double mutants frontal cortex

| PRJNA598644 | ENA

The human gene FOXM1 promotes cortical folding and improves learning and memory ability in mice [P0]

Project description:Purpose: To gain further insight into how FOXM1 causes generation of cortical expansion and folding in mice, single cell RNA-seq was used to analyze the cell type changes resulting from the cerebral cortices of P0 FOXM1 conditional knock in mice and littermate wild-type. Methods: Single cell isolation was prepared from P0 telencephalic tissue of wild-type (WT) and FOXM1f/+;Nestin-Cre mice. The fragmented cDNAs containing barcode and UMI sequences was built the library. Agilent 2100 Bioanalyzer was used to quality controlled and quantified. Single cell RNA-sequencing analysis was used by the Illumina HiSeq 2500 platform in Annoroad Genomics. Results: Clustering and labeling of these cells visualized on a t-SNE plot between the wild-type (WT) and FOXM1f/+;Nestin-Cre mice brain cortex. Conclusions: Single cell RNA-seq data would provide an overall understanding how FOXM1 gene contribute to generation of cortical expansion and folding in mice.

2020-02-26 | GSE145934 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data