Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Whole transcriptome sequencing of Npas3+/- and WT cortex at P0 stage

ABSTRACT: Whole transcriptome sequencing of Npas3+/- and WT cortex at P0 stage

PROVIDER: PRJNA744260 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15054752_1.fastq.gz	Fastqsanger.gz
	SRR15054752_2.fastq.gz	Fastqsanger.gz
	SRR15054753_1.fastq.gz	Fastqsanger.gz
	SRR15054753_2.fastq.gz	Fastqsanger.gz
	SRR15054754_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Whole transcriptome sequencing of Npas3+/- and WT cortex at P0 stage

Project description:Purpose: To gain insight into the impact of NPAS3 deficiency on astrogenesis in mice, RNA-seq was used to analyze the genome-wide changes resulting from Npas3+/- and WT cortex at P0 stage. Methods: Total RNA was extracted from Npas3+/- and WT cortex at P0 stage. Then, total RNA was quantified and quality controlled by Agilent Bioanalyzer 2100 system. After cDNA library construction, the library preparations were sequenced on an Illumina NovaSeq 6000 platform in Novogene. Results: At significance cutoffs corresponding to FDR < 0.05, a total of 625 DEGs were identified in Npas3+/- mice. Conclusions: Our results show that Npas3+/- mice exhibit defective astrogenesis and abnormal synapse function.

2022-07-19 | GSE179608 | GEO

NPAS3-regulated astrocyte mitochondria bioenergetics is required for cognition

Project description:The basic helix-loop-helix (bHLH) transcription factor neuronal PAS domain protein 3 (NPAS3) provides transcriptional regulation of metabolic pathways, and despite its name is highly expressed in astrocytes. NPAS3 variants have been associated with cognitive dysfunction in several neuropsychiatric conditions, but the underlying brain cell type-specific mechanisms remain obscure. Here, we report that NPAS3 is a key regulator of mitochondria bioenergetics in astrocytes. Selective deletion of Npas3 in mature murine astrocytes decreases expression of Slc25a18 and its protein product, mitochondrial glutamate carrier 2, leading to reduced oxidative phosphorylation and lactate production in astrocytes. This deficit subsequently causes reduced excitability, dendritic spine density, and dendritic arborization of medial prefrontal cortex pyramidal neurons. Mice with Npas3-deficient cortical astrocytes exhibit impaired trace fear conditioning, which is rescued by lactate treatment. Thus, the present study demonstrates a mechanistic link between NPAS3-dependent astrocyte mitochondria bioenergetics and cognitive function and provides insights for glia-targeting treatment of cognitive dysfunction in neuropsychiatric disease.

2026-05-04 | GSE274921 | GEO

Whole transcriptome sequencing of mouse cortical astrocytes (DIV 14).

Project description:To gain futher insight into the Npas3 regulatory network in astrocytes, RNA-seq was used to analyze the genome-wide changes resulting from the days in vitro (DIV) 14 astrocytes of P0 wild-type (WT) mice and littermate NPAS3-/- mice. Total RNA was extracted from DIV 14 astrocytes of P0 Npas3-/- and WT mice. Then, total RNA was quantiﬁed and quality controlled by Agilent Bioanalyzer 2100 system. After cDNA library construction, the library preparations were sequenced on an Illumina NovaSeq 6000 platform in Novogene.

2022-07-19 | GSE179602 | GEO

NPAS3-HEK293-standardcond-Illumina

Project description:NPAS3 or truncated NPAS3 overexpressed HEK293 cells or control HEK293 cells were collected at 24 hours after gene overexpression.Total RNA from alll samples was extracted.Biotinylated cRNA was prepared using the Illumina total Prep RNA application Kits.Hybridization, washing and scanning were performed according to the Illumina BeadStation 500* manual (revision C)

2011-10-03 | E-TABM-1078 | biostudies-arrayexpress

RNA-sequencing experiments comparing P0 control and RXRG/RARb double mutants frontal cortex

Project description:To understand the functional significance of RA signaling through the RXRG/RARB heterodimer in the developing cortex, we performed RNA-seq experiments comparing different regions of the P0 mouse frontal cortex (mPFC, secondary motor cortex (MOs)/ primary motor cortex (MOp), and OFC ) in Rarb and Rxrg dKO and WT littermate controls.

2021-01-01 | GSE142851 | GEO

Identification of NPAS3 cistrome in the mouse cortical astrocytes (DIV 14).

Project description:Genome-wide binding sites of NPAS3 were identified in mouse cortical astrocytes (DIV 14).

2022-07-19 | GSE179592 | GEO

Transcriptomic analysis of the cortex of ASPM Knockout Macaca fascicularis at P0

Project description:Transcriptomic analysis of the cortex of ASPM Knockout Macaca fascicularis at P0

| PRJNA526884 | ENA

RNA-sequencing experiments comparing P0 control and RXRG/RARb double mutants frontal cortex

Project description:RNA-sequencing experiments comparing P0 control and RXRG/RARb double mutants frontal cortex

| PRJNA598644 | ENA

P0 kidney samples

Project description:P0 kidney samples