ABSTRACT: Multi-omics strategy uncovered the probiotic Lactiplantibacillus plantarum P9 regulated the gut microbial metabolites and alleviated inflammatory response in a pesticide exposure cohort
Project description:In this study we conducted a randomized, double-blind, placebo-controlled trial to evaluate the effects of administering probiotic mixtures containing Lactobacillus casei Zhang, Bifidobacterium lactis V9, and Lactobacillus plantarum P9 in patients with GERD. Patients were randomized into probiotic and placebo groups . All patients were treated with rabeprazole tablets and simultaneously received either probiotic or placebo for 8 weeks, and then administrated with probiotic or placebo alone for 4 weeks. Fecal samples from patients were collected and analyzed by untargeted metabolomics at weeks 0, 4, 8, 12.
Project description:The study was conducted on a model of Lactiplantibacillus plantarum, one of the most studied species widely used in the food industry as a probiotic microorganism and/or microbial starter culture. As a result of step-by-step selection from the L. plantarum 8p-a3 strain isolated from the «Lactobacterin» probiotic, the L. plantarum 8p-a3-Clr-Amx strain was obtained, showing increased resistance, compared with the parent strain, to amoxicillin-clavulanic acid (MIC 20 mcg/ml) and clarithromycin (MIC 10 mcg/ml). The L. plantarum strain DMC-S1 was isolated from the intestine of Drosophila melanogaster Canton-S line. Extracellular vesicles of this bacterium can play a significant role in the drug-resistance development and host-microbe interactions.
Project description:The study evaluates the effect of Lactiplantibacillus plantarum IMC 510® supplementation on anthropometric and biochemical parameters, GM composition and gastrointestinal and general symptoms of overweight/obese subjects.
Project description:Strains of Lactiplantibacillus plantarum treated with lithium chloride to extract non-covalently bound cell wall proteins that were identified by mass spectrometry proteomic analysis.
Project description:Using Drosophila we identified a glia-secreted antimicrobial peptide IM33, which alters gut microbiota to modulate sleep. To understand how the gut commensal Lactiplantibacillus plantarum functions in the brain to regulate sleep, we performed single-cell RNA sequencing.
Project description:The ability of Lactiplantibacillus plantarum LOC1 and LOC3, originally isolated from fresh tea leaves, to modulate the response of murine macrophages to the activation of Toll-like receptor 4 (TLR4) by the stimulation with lipopolysaccharide (LPS) was evaluated.
