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ABSTRACT:
PROVIDER: PRJNA744318 | ENA |
REPOSITORIES: ENA
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Project description:Prunus mongolica is a relict xerophytic shrub with ecological importance for desert ecosystem stability and vegetation restoration in arid and semi-arid regions. However, the physiological and molecular mechanisms underlying its responses to combined alkali-salt and cadmium stress remain unclear. This study aimed to investigate the regulatory role of exogenous gamma-aminobutyric acid (GABA) in improving stress tolerance in Prunus mongolica under alkali-salt stress, cadmium stress, and their combined stress. Seedlings were subjected to eight treatments, including control, GABA alone, alkali-salt stress, cadmium stress, combined alkali-salt and cadmium stress, and the corresponding GABA-treated stress groups. Germination traits, seedling growth, physiological indices, antioxidant responses, osmotic adjustment, photosynthetic pigments, and transcriptomic profiles were analyzed to evaluate the effects of GABA at multiple biological levels. Alkali-salt, cadmium, and combined alkali-salt and cadmium stress markedly inhibited seed germination and seedling growth, with the combined stress causing the most severe damage. Stress treatments promoted reactive oxygen species accumulation, lipid peroxidation, photosynthetic inhibition, and reductions in water status and root activity. Exogenous GABA alleviated these stress-induced effects by improving germination performance, seedling growth, chlorophyll accumulation, root activity, and relative water content, while reducing oxidative damage. GABA also enhanced osmotic adjustment by increasing proline and soluble sugar contents and strengthened antioxidant defense through the activation of superoxide dismutase, peroxidase, catalase, ascorbate peroxidase, and glutathione reductase. Transcriptome analysis showed that GABA modulated stress-responsive genes associated with MAPK signaling, plant hormone signal transduction, plant-pathogen interaction, peroxisome, glutathione metabolism, photosynthesis, carbon metabolism, and other primary metabolic processes. These results suggest that exogenous GABA enhances stress tolerance in Prunus mongolica by coordinating redox homeostasis, osmotic regulation, stress signaling, photosynthetic recovery, and metabolic reprogramming under single and combined alkali-salt and cadmium stresses.
2026-07-03 | GSE337475 | GEO
Project description:Purpose: The goal of this study is to provided a comprehensive genomic information for functional genomic studies in Q. mongolica. Methods:The Quercus mongolica leaves were generated by deep sequencing, using Illumina Hiseq 4000. The high-quality reads were obtained by removing the reads that contained adaptor contamination, low quality bases and undetermined bases.The transcriptome were de novo assembly. Results:A total of 52934562 raw reads were obtained from Illumina sequencing platform. After filtering out the low quality reads, we obtained 52076914 clean reads, which assembled into 39130 transcripts with a mean length of 742 bp and GC content of 42.12%, and 24196 unigenes with a mean length of 732 bp and GC content of 42.34%, based on Trinity assembly platform. Conclusions:RNA-Seq was applied to polyadenylate-enriched mRNAs from leaves of Q. mongolica to obtain the transcriptome. De novo assembly was then applied followed by gene annotation and functional classification. The SSRs and SNPs were also obtained using assembled transcripts as reference sequences. The results of this study lay the foundation for further research on genetic diversity of Quercus.
2019-01-29 | GSE125799 | GEO
Project description:Prunus mongolica Genome sequencing and assembly
| PRJNA930130 | ENA
Project description:Prunus mongolica Transcriptome or Gene expression
| PRJNA248054 | ENA
Project description:Mycorrhizal Prunus mongolica Transcriptome or Gene expression
| PRJNA271925 | ENA