Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A customized synthetic gene circuit selectively engages an immune-related transcriptional program in p53-negative LLC cells

ABSTRACT: A customized synthetic gene circuit selectively engages an immune-related transcriptional program in p53-negative LLC cells

PROVIDER: PRJNA745163 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR15085158_1.fastq.gz	Fastqsanger.gz
	SRR15085158_2.fastq.gz	Fastqsanger.gz
	SRR15085159_1.fastq.gz	Fastqsanger.gz
	SRR15085159_2.fastq.gz	Fastqsanger.gz
	SRR15085160_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

A customized synthetic gene circuit selectively engages an immune-related transcriptional program in p53-negative LLC cells

Project description:Purpose: Cancer immunotherapy has shown promising results in both mouse models and clinical trials. However, to specifically reprogram the immune microenvironment of advanced tumor lesions remains a huge challenge. Therefore, we assembled a synthetic gene circuit aimed at specifically targeting p53-negative LLC cells, but not their p53+ derivatives, for immune activation. Methods: Firstly, we established an LLC-derived knock-in line (p53+), where the WT p53 coding region was re-introduced into the endogenous locus to rescue the bi-allelic point mutations (a nonsense mutation at E32, and a R334P mutation) in the parental LLC cells. Next, both the parental LLC cells and p53+ LLC cells were introduced with the AND-NOT gene circuits which contains the tumor-specific survivin promoter-driven dCas9 (Psuv-dCas9), U6 promoter-driven Ifng-activating sgRNA (or control sgRNA), and p53-responsive promoter PM2-driven AcrIIA4max. 48 hours after transfection, the total RNA was isolated and subjected to RNAseq services. Results: The p53+ LLC cell showed differences of hundreds of genes compared with the parental LLC cells. This group of genes contain a number of classical p53 targets. Notably, the logic circuit drove selective induction of a different gene set in the parental, but not the p53+ cells. Importantly, the vast majority of the circuit-induced genes are known downstream targets of IFN, confirming the specificity of a CRISPRa-driven transcriptional rewiring. Conclusion: Overall, this dataset provides evidence that the AND-NOT gene circuit selectively targets p53-negative LLC cells, but not their p53+ derivatives, and subsequently programs a highly specific immune-rewiring output.

2022-02-23 | GSE179814 | GEO

Gene expression profiling of mature dendritic cells from mice s. c. treated with GM-CSF-gene transduced LLC cells (LLC/SeV/GM) or control cells (LLC, LLC/SeV/GFP)

Project description:Irradiated granulocyte macrophage-colony stimulating factor (GM-CSF)-transduced autologous tumor cells induce substantial antitumor immunity through the maturation and migration of dendritic cells (DCs). However, little is known about the key molecules involved in GM-CSF-sensitized DCs (GM-DCs) in tumor draining lymph nodes (TDLNs). We initially confirmed that mice subcutaneously injected with poorly immunogenic syngeneic Lewis lung carcinoma (LLC) cells transduced with Sendai virus encoding GM-CSF (LLC/SeV/GM) significantly rejected the tumor growth. Using microarray expression profiling, we obtained a large number of gene expression data files from GM-DCs and control DCs in TDLNs, and subjected them to network-based cluster analysis and unexpectedly unraveled the expression levels of type I IFNs-related genes specifically expressed in plasmacytoid DCs (pDC) were robustly up-regulated in GM-DCs. In vivo depletion assay showed that pDC-depleted mice treated with subcutaneous LLC/SeV/GM cells abrogated the antitumor effects observed in control mice. Moreover combination use of imiquimod for TLR7 triggering on pDC with irradiated LLC/SeV/GM cells induced a significant therapeutic antitumor effect with marked induction of CD9+ pDC with antitumor phenotype, whereas other control mice groups had only minimal to-modest antitumor responses, implicating that this combined vaccine strategy using imiquimod could be promising for improvement of GM-CSF-induced antitumor immunity. Mouse GM-CSF induced gene expression in mature dendritic cells in tumor draining lymph nodes from C57/BL6N female mouse was measured at 2 days after s.c. tumor challenge with GM-CSF gene-transduced LLC cells (LLC/SeV/GM) or control cells (LLC, LLC/SeV/GFP).

2013-09-30 | E-GEOD-43169 | biostudies-arrayexpress

Stop Mole LLC

Project description:Stop Mole LLC

| PRJNA1097484 | ENA

Transcription profiling of Mus musculus PBS vs IL-5 trerated LLC cells, and LLC vs B16 cells at baseline conditions

Project description:PBS and Il-5 (22 pM) treated LLC and B16 cells were harvested after 24 hours, for comparison between: 1) PBS and IL-5 trerated LLC cells, and 2) btween LLC and B16 cells at baseline conditions.

2010-12-13 | E-MEXP-2490 | biostudies-arrayexpress

Murine cells: MSC+LLC-serum-exosome vs LLC-serum-exosome

Project description:Murine cells: MSC+LLC-serum-exosome vs LLC-serum-exosome

| PRJNA490558 | ENA

Murine cells: MSC+LLC-serum-exosome vs LLC-serum-exosome

Project description:Exosomal miRNAs including 86 exosomal miRNAs were significantly increased and 354 exosomal miRNAs were significantly decreased in the co-injection group compared to LLC injection alone through expression profiling of a total of 1903 genes.

2019-04-02 | GSE119887 | GEO

Gene expression profiling of mature dendritic cells from mice s. c. treated with GM-CSF-gene transduced LLC cells (LLC/SeV/GM) or control cells (LLC, LLC/SeV/GFP)

2013-09-30 | GSE43169 | GEO

RNA sequencing in LLCs from co-injected mice or LLC injection alone.

Project description:we analyzed transcriptomic profiles in LLC cells isolated from primary cancer sites. C57BL/6 mice were subcutaneously injected with LLC-RFP with or without BMSCs.

2019-04-02 | GSE120349 | GEO

Expression data from the subclones of Lewis Lung Carcinoma (LLC) cells

Project description:Medium conditioned by LLC cells stimulates thermogenic gene expression when added onto primary adipocytes. We generated single cell colonies from parental LLC cells. Media conditioned by the subclones stimulated thermogenic gene expression in primary adipocytes at varying degrees. Subclones 2, 3, 6 and 28 produce significantly larger amount of thermogenic activity than the subclones 18, 19, 23 and 24. We compared gene expression profiles of these subclones to identify secreted factors enriched in the more thermogenic clones. LLC subclones were cultured under conditioned medium preparation conditiones and total RNA was extracted from biological replicates.

2014-07-13 | E-GEOD-57797 | biostudies-arrayexpress

Pseudomonas sp. LLC-1

Project description:Pseudomonas sp. LLC-1 genome sequencing project

| PRJNA314471 | ENA