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RNA-seq analysis of freshly isolated and ex-vivo cultured human cord blood CD34+ cells

ABSTRACT: RNA-seq analysis of freshly isolated and ex-vivo cultured human cord blood CD34+ cells

PROVIDER: PRJNA745928 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15101753.fastq.gz	Fastqsanger.gz
	SRR15101754.fastq.gz	Fastqsanger.gz
	SRR15101755.fastq.gz	Fastqsanger.gz
	SRR15101756.fastq.gz	Fastqsanger.gz
	SRR15101757.fastq.gz	Fastqsanger.gz

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RNA-seq analysis of freshly isolated and ex-vivo cultured human cord blood CD34+ cells

Project description:RNA-seq analysis was performed to compare differentially expressed genes in freshly isolated and ex-vivo cultured human cord blood CD34+ cells. Mitochondrion related genes are upregulated in CD34+ hematopoietic stem and progenitor cells upon ex vivo culture. In vivo transplantation experiments demonstrate that stemness of CD34+ cells is significantly decreased due to oxidative stress induced by ex vivo culture.

2021-09-30 | GSE179928 | GEO

Gene expression data comparing HIV exposed and HIV unexposed freshly isolated umbilical cord blood derived CD34+ haematopoietic stem/progenitor cells

Project description:Umbilical cord blood (UCB) is a donor source for haematopoietic stem cell transplantation but only HIV unexposed and uninfected cord blood has been used to date. HIV exposed and uninfected cord blood could potentially be considered as a donor source in HIV endemic countries, provided the functioning of these CD34+ Haematopoietic stem cells (HSPCs) is comparable to HIV unexposed cells. We performed gene expression analysis on umbilical cord blood CD34+ HSPCs to determine if any differences exist between HIV unexposed and HIV exposed cohorts with mothers taking newer antiretroviral drugs.

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Lin-CD34+ cord-blood cells

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Mitochondrial activity linked to CD34+CD90+EPCR+ phenotype determines the functional fitness of ex vivo expanded HSCs

Project description:We report the transcritpome of purified CD34+ cells from cultures initiated with cord blood CD34+ hematopoietic stem cells that were expanded ex vivo in Stemline II media supplemented with a cytokine cocktail in the presence or absence of valproic acid for 6 days .

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Gene expression data comparing HIV exposed and HIV unexposed freshly isolated umbilical cord blood derived CD34+ haematopoietic stem/progenitor cells

Project description:Gene expression data comparing HIV exposed and HIV unexposed freshly isolated umbilical cord blood derived CD34+ haematopoietic stem/progenitor cells

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Highly purified human cord blood-derived CD34+/- hematopoietic stem cells

Project description:Global gene expressions of human cord blood-derived 18Lineage-negative (18Lin-)CD34+CD38-CD133+GPI-80+ cells (CD34+ HSCs), 18Lin-CD34-CD133+GPI-80+ cells (CD34- HSCs) and 18Lin-CD34+CD133- cells (non-HSCs) were analyzed. Results provide an insight into the molecular mechanisms underlying the self-renewal, maintenance and differentiation of human cord blood-derived CD34+/- HSCs.

2017-06-23 | GSE100354 | GEO

RNAseq of freshly isolated and ex vivo expanded peripheral blood NK cells

Project description:RNAseq of freshly isolated and ex vivo expanded peripheral blood NK cells

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AML1-ETO transduced human cord blood cells, CD34 selected, compared to normal cord blood cells, CD34 selected

Project description:AML1-ETO expression in normal human umbilical cord blood CD34+ cells leads to long-term proliferation of an early self-renewing primitive progenitor cell with multilineage potential and stem cell ability, but these cells do not induce leukemia in immunodeficient mice. This comparative microarray study was initiated to determine the differences in the transcriptome of AML-ETO-expressing CD34+ cells after extended culture in vitro, using normal cord blood cells expanded for 6-8 weeks in vitro and subsequently purified for the CD34+ population as the control comparison. Keywords: Disease state analysis; comparison of changes in transcriptome due to long-term AML1-ETO expression in normal human hematopoietic CD34+ progenitor cells

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Expression data from human CD34+ HPC subpopulations isolated from umbilical cord blood (Haddad et al. Blood 104:3918, 2004)

Project description:We used microarrays to analyze the gene expression profile of CD34+CD45RA+CD7+, CD34+CD45RA+CD10+CD19- and CD34+CD45+CD7-CD10-CD19- HPCs isolated from umbilical cord blood CD34+CD45RA+CD7+(CD10-) and CD34+CD45RA+CD10+(CD7-CD19-) HPCs correspond respectively to prothymocytes and early pre-proB precursors. CD34+CD45RA+CD7-CD10-CD19- HPCs correspond to lympho-granulo-macrophagic precursors

2011-05-25 | GSE29522 | GEO

DNA methylation profiles of freshly isolated CD34+ cells and upon expansion on either tissue culture plastic (TCP) or mesenchymal stromal cells (MSCs)

Project description:Hematopoietic stem and progenitor cells (HPCs) can be maintained in vitro, but the vast majority of their progeny loses “stemness” during culture. In this study, we have analyzed DNA methylation (DNAm) profiles of freshly isolated CD34+ cells and upon expansion on either tissue culture plastic (TCP) or mesenchymal stromal cells (MSCs). DNAm profiles of expanded CD34+ versus CD34- subsets reflected hematopoietic differentiation, whereas culture on TCP or MSCs had little impact. Notably, all cultured HPCs - even those which remained CD34 positive - acquired significant DNA-hypermethylation, particularly in up-stream promoter regions, shore-regions of CpG islands, and binding sides for PU.1 and RUNX1. Our results point to a coordinated epigenetic process which needs to be controlled to enhance self-renewal of HPCs in vitro.

2013-12-03 | GSE40799 | GEO

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