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Transcriptome analysis of LPS stimulated PECs (WT and Zc3h12a S513A)

ABSTRACT: Transcriptome analysis of LPS stimulated PECs (WT and Zc3h12a S513A)

PROVIDER: PRJNA746300 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15123701_1.fastq.gz	Fastqsanger.gz
	SRR15123701_2.fastq.gz	Fastqsanger.gz
	SRR15123702_1.fastq.gz	Fastqsanger.gz
	SRR15123702_2.fastq.gz	Fastqsanger.gz
	SRR15123703_1.fastq.gz	Fastqsanger.gz

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Project description:Lipopolysaccharide (LPS), a Toll-like receptor (TLR) 4 ligand, induces the expression of various genes including proinflammatory cytokines, and the expression is modified by the presence of Zc3h12a. We used microarrays to examine influence of Zc3h12a deficiency in LPS-inducible gene expression. Experiment Overall Design: Peritoneal macrophages from wild-type and Zc3h12a-/- mice were stimulated with LPS for 0, 1, 2 and 4 hours, followed by RNA extraction. Then hybridization on affymetrix microarrays was performed.

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