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ABSTRACT: Histophilus somni isolates from Bovine Respiratory Disease samples

PROVIDER: PRJNA746355 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15128700_1.fastq.gz	Fastqsanger.gz
	SRR15128700_2.fastq.gz	Fastqsanger.gz
	SRR15128701_1.fastq.gz	Fastqsanger.gz
	SRR15128701_2.fastq.gz	Fastqsanger.gz
	SRR15128702_1.fastq.gz	Fastqsanger.gz

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Protein composition of the biofilm matrix and the outer membrane vesicles (OMVs) during iron-sufficient and iron-restricted growth.

Project description:There is limited efficacy in vaccines currently available to prevent some animal diseases of bacterial origin, such as bovine respiratory disease due to Histophilus somni. Protective efficacy can potentially be improved if bacterial antigens expressed in the host are included in vaccines. During H. somni infection in the bovine host, biofilms become established and the availability of essential iron is restricted. To investigate potential proteins as protective antigens, the protein composition of spontaneously released outer membrane vesicles (OMVs) during iron-sufficient and iron-restricted growth, and the proteins expressed in the biofilm matrix were analyzed and compared. Proteomic analysis revealed a dramatic physiological change in H. somni as it transitioned from the planktonic form to the biofilm mode of growth. All transferrin-binding proteins (Tbp) previously identified in H. somni were detected in the OMVs, suggesting that OMVs could induce antibodies to these proteins. Two TbpA-like proteins and 7 total proteins were present in the OMVs only when iron was restricted, indicating that the expression of these Tbp was differentially regulated. More proteins associated with quorum-sensing (QS) signaling were detected in the biofilm matrix compared with proteins in the OMVs, supporting a link between QS and biofilm formation. Proteins ACA31267.1 (OmpA) and ACA32419.1 (TonB-dependent receptor) were present in the OMV and biofilm matrix, and predicted to be potential protective antigens using an immuno-bioinformatic approach. Overall, the results support the development of novel vaccines that contain OMVs obtained from bacteria grown to simulate the in vivo environment, and possibly biofilm matrix, to prevent diseases due to bacterial pathogens.

2025-05-15 | PXD061987 | Pride

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